Rhizobium miluonense sp. nov., a symbiotic bacterium isolated from Lespedeza root nodules

• Published in: International journal of systematic and evolutionary microbiology Vol. 58; no. 6; pp. 1364 - 1368
• Main Authors: Gu, Chun Tao, Wang, En Tao, Tian, Chang Fu, Han, Tian Xu, Chen, Wen Feng, Sui, Xin Hua, Chen, Wen Xin
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.06.2008; Society for General Microbiology
• Subjects: Agronomy. Soil science and plant productions; Bacteria; Bacterial Proteins - chemistry; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; China; DNA, Bacterial - analysis; DNA, Ribosomal Spacer - analysis; Economic plant physiology; Electrophoresis, Polyacrylamide Gel; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Lespedeza; Lespedeza - microbiology; Microbiology; Molecular Sequence Data; Nucleic Acid Hybridization; Phaseolus vulgaris; Phenotype; Plant Roots - microbiology; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Rhizobium; Rhizobium - classification; Rhizobium - genetics; Rhizobium - isolation & purification; Rhizobium - physiology; RNA, Ribosomal, 16S - chemistry; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Species Specificity; Symbiosis; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...); Systematics; China; Symbiosis; Taxonomy; Lespedeza; Plant nodule; Rhizobium; Symbiont; Plant; Leguminosae; Dicotyledones; Angiospermae; Bacteria; Spermatophyta; New species; Rhizobiaceae
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.65661-0

1 Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture/Department of Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100094, China 2 Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México DF 11340, Mexico Correspondence Wen Xin Chen wenxin_chen{at}263.net Seventeen strains of lespedeza rhizobia, a previously defined novel group, were further characterized by comparison with phylogenetically related species using a polyphasic approach, including SDS-PAGE of whole-cell proteins, PCR-based restriction fragment length polymorphism analysis of ribosomal intergenic spacer sequences, determination of DNA G+C contents, DNA–DNA hybridization and analysis of phenotypic features. Based on data obtained in the present and previous studies, a novel species, Rhizobium miluonense sp. nov., is proposed for these strains. The type strain of R. miluonense is CCBAU 41251 T (=LMG 24208 T =HAMBI 2971 T ). Strain CCBAU 41251 T could form ineffective nodules on Phaseolus vulgaris in addition to its original host Lespedeza chinensis . Abbreviations: IGS, intergenic spacer; RFLP, restriction fragment length polymorphism The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Rhizobium miluonense CCBAU 41251 T is EF061096 . Phenotypic characteristics of strains of Rhizobium miluonense sp. nov. are given in Supplementary Table S1, which is available with the online version of this paper.