Determination of plasma testosterone by mass fragmentography using [3,4- 13C]testosterone as an internal standard
The combination of glass capillary gas chromatography—mass spectrometry is especially suitable for the recognition of compounds. The use of [3,4- 13C]testosterone as internal standard, mass fragmentography and isotope ratio measurement have been applied to the quantitative determination of testoster...
Saved in:
Published in | Journal of chromatography Vol. 339; no. 2; pp. 233 - 242 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
01.01.1985
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | The combination of glass capillary gas chromatography—mass spectrometry is especially suitable for the recognition of compounds. The use of [3,4-
13C]testosterone as internal standard, mass fragmentography and isotope ratio measurement have been applied to the quantitative determination of testosterone in plasma. This paper describes the method, using
tert.-butyldimethylsilylmethoxime and di-heptafluorobutyrate derivatives. The calibration graph in isotopic dilution is examined. The results obtained are compared with the results obtained by radioimmunoassay. The sensitivity of the method is judged from the lower limit of detection: 4.5 pg. The precision, and inter- and intra-assay are calculated. |
---|---|
ISSN: | 0378-4347 |
DOI: | 10.1016/S0378-4347(00)84650-2 |