Protective Effects of a Novel Lactobacillus brevis Strain with Probiotic Characteristics against Staphylococcus aureus Lipoteichoic Acid-Induced Intestinal Inflammatory Response

• Published in: Journal of microbiology and biotechnology Vol. 32; no. 2; pp. 205 - 211
• Main Authors: Kim, Won-Ju, Hyun, Jun-Hyun, Lee, Na-Kyoung, Paik, Hyun-Dong
• Format: Journal Article
• Language: English
• Published: Korea (South) The Korean Society for Microbiology and Biotechnology 28.02.2022; 한국미생물·생명공학회
• Subjects: Levilactobacillus brevis; Lipopolysaccharides; Probiotics; Research article; Staphylococcus aureus; Teichoic Acids; 생물학; ERK signaling; anti-inflammatory; Probiotics; Akt signaling; HT-29 cell
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.2110.10034

Probiotics can effectively modulate host immune responses and prevent gastrointestinal diseases. The objective of this study was to investigate the probiotic characteristics of KU15152 isolated from kimchi and its protective potential against intestinal inflammation induced by lipoteichoic acid (aLTA). KU15152 exhibited a high survival rate in artificial gastric and bile environments. Additionally, the adhesion capability of the strain to HT-29 cells was higher than that of GG. KU15152 did not produce harmful enzymes, such as β-glucuronidase, indicating that it could be used as a potential probiotic. The anti-inflammatory potential of KU15152 was determined in HT-29 cells. Treatment with KU15152 suppressed the production of interleukin-8 without inducing significant cytotoxicity. The downregulatory effects of KU15152 were involved in the suppression of nuclear factor-kappa B activation mediated by the extracellular signal-regulated kinase and Akt signaling pathways. Collectively, these data suggest that KU15152 can be used in developing therapeutic and prophylactic products to manage and treat aLTA-induced intestinal damage.