Transcriptome Analysis Unveils Gln3 Role in Amino Acids Assimilation and Fluconazole Resistance in Candida glabrata

• Published in: Journal of microbiology and biotechnology Vol. 31; no. 5; pp. 659 - 666
• Main Authors: Pérez-de Los Santos, Francisco J, García-Ortega, Luis Fernando, Robledo-Márquez, Karina, Guzmán-Moreno, Jesús, Riego-Ruiz, Lina
• Format: Journal Article
• Language: English
• Published: Korea (South) The Korean Society for Microbiology and Biotechnology 28.05.2021; 한국미생물·생명공학회
• Subjects: Amino Acids - biosynthesis; Ammonium Compounds - metabolism; Antifungal Agents - metabolism; Antifungal Agents - pharmacology; ATP-Binding Cassette Transporters - genetics; Candida glabrata - drug effects; Candida glabrata - genetics; Candida glabrata - metabolism; Candida glabrata - physiology; Catabolite Repression; Drug Resistance, Fungal - drug effects; Drug Resistance, Fungal - genetics; Fluconazole - metabolism; Fluconazole - pharmacology; Fungal Proteins - genetics; Fungal Proteins - metabolism; GATA Transcription Factors - genetics; GATA Transcription Factors - metabolism; Gene Expression Profiling; Gene Expression Regulation, Fungal; Mutation; Research article; 생물학; ABC transporters; Candida glabrata; nitrogen catabolite repression; Gln3; fluconazole resistance
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.2012.12034

After , is one of the most common fungal species associated with candidemia in nosocomial infections. Rapid acquisition of nutrients from the host is important for the survival of pathogens which possess the metabolic flexibility to assimilate different carbon and nitrogen compounds. In , nitrogen assimilation is controlled through a mechanism known as Nitrogen Catabolite Repression (NCR). NCR is coordinated by the action of four GATA factors; two positive regulators, Gat1 and Gln3, and two negative regulators, Gzf3 and Dal80. A mechanism in similar to NCR in has not been broadly studied. We previously showed that in , Gln3, and not Gat1, has a major role in nitrogen assimilation as opposed to what has been observed in in which both factors regulate NCR-sensitive genes. Here, we expand the knowledge about the role of Gln3 from through the transcriptional analysis of BG14 and strains. Approximately, 53.5% of the detected genes were differentially expressed (DEG). From these DEG, amino acid metabolism and ABC transporters were two of the most enriched KEGG categories in our analysis (Up-DEG and Down-DEG, respectively). Furthermore, a positive role of Gln3 in AAA assimilation was described, as was its role in the transcriptional regulation of . Finally, an unexpected negative role of Gln3 in the gene regulation of ABC transporters and and its associated transcriptional regulator was found. This observation was confirmed by a decreased susceptibility of the strain to fluconazole.