Ultrastructural localization of adenylate cyclase in chicken bone cells

• Published in: The journal of histochemistry and cytochemistry Vol. 37; no. 11; pp. 1705 - 1709
• Main Authors: Yamamoto, T, Gay, CV
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA Histochemical Soc 01.11.1989; SAGE Publications; Histochemical Society
• Subjects: Adenylyl Cyclases - metabolism; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Bone and Bones - cytology; Bone and Bones - enzymology; Bone and Bones - ultrastructure; Chickens; Colforsin; Cyclic AMP - metabolism; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; Histocytochemistry; Lyases; Microscopy, Electron; Osteoblasts - metabolism; Osteoblasts - ultrastructure; Osteoclasts - metabolism; Osteoclasts - ultrastructure
• ISSN: 0022-1554; 1551-5044
• DOI: 10.1177/37.11.2553803

We investigated adenylate cyclase distribution in 6-day-and 3-week-old calvariae and in 6-day-old long bone metaphyses from chickens. Reaction product distribution was on the plasma membrane of osteoblasts, pre-osteoblasts, and forming osteocytes which contacted one another. Osteoclasts and mature osteocytes lacked reaction product. Six-day calvariae reacted less intensely than the other two tissues. In controls, reaction product was markedly diminished or eliminated by removal of forskolin or substrates, or by addition of the inhibitor 2',5'-dideoxyadenosine. The results indicate the importance of cyclic AMP in osteoblast regulation. Osteocytes and osteoclasts may involve alternate mechanisms as major regulatory systems.