Trichomonas vaginalis: Identification of a triacylglycerol acylhydrolase

• Published in: Experimental parasitology Vol. 111; no. 4; pp. 260 - 263
• Main Authors: Carvalho, Sandra Elisa, Freitas, Daniel, Murad, André Melro, Franco, Octávio Luiz, Simões-Barbosa, Augusto
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.12.2005; Elsevier
• Subjects: Amino Acid Sequence; Animals; Basic Local Alignment Search Tool; Biological and medical sciences; BLAST; Conserved Sequence; FFAS3; Fold and Function Assignment System; Fundamental and applied biological sciences. Psychology; gene encoding a putative triacylglycerol acylhydrolase protein in Trichomonas vaginalis; Gene Expression Regulation, Enzymologic; Hemolysis; Hemolysis - physiology; IPTG; isopropyl- d-thiogalactopyranoside; Life cycle. Host-agent relationship. Pathogenesis; Lipase; Lipase - chemistry; Lipase - genetics; Lipase - isolation & purification; Luria broth; Molecular Sequence Data; Pathogenesis; Protein Conformation; Protein Structure, Secondary; Protozoa; Protozoan Proteins - chemistry; Protozoan Proteins - genetics; Protozoan Proteins - isolation & purification; Triacylglycerol acylhydrolase; Trichomonas vaginalis; Trichomonas vaginalis - enzymology; Trichomonas vaginalis - genetics; Trichomoniasis; TvLIP; Trichomonas vaginalis; Hemolysis; Trichomoniasis; Pathogenesis; Triacylglycerol acylhydrolase; LB; TvLIP; IPTG; FFAS3; Lipase; BLAST; Fold; Protozoa; Protozoal disease; isopropyl-D-thiogalactopyranoside; LB; Fold and Function Assignment System; TvLIP; Parasite; gene encoding a putative triacylglycerol acylhydrolase protein in Trichomonas vaginalis; Trichomonas vaginalis; Hemolysis; Triacylglycerol acylhydrolase; Lipase; Trichomoniasis; Pathogenesis; IPTG; Parasitosis; Trichomonadida; Protein; Infection; Assignment; Alignment; Luria broth; BLAST; Gene; Triacylglycerol; Basic Local Alignment Search Tool; FFAS3
• ISSN: 0014-4894; 1090-2449
• DOI: 10.1016/j.exppara.2005.08.007

This work describes the identification of a triacylglycerol lipase named TVLip directly onto blood-LB-agar plates by hemolytic screening of a Trichomonas vaginalis cDNA expression library. Sharing significant similarity in the primary sequence with other lipases, the theoretical 3D structure of the TVLip was resolved. The structure reveals the predictive conserved characteristics of other lipases from EC3.1.1.3 group, although presenting one aminoacid change in the catalytic triad Ser-His-Asp. Finally, analysis of Northern blot indicates that the expression of the TVLip gene is up-regulated by iron.