Entamoeba histolytica: Apoptosis induced in vitro by nitric oxide species
Apoptosis has been described in some parasites like Leishmania, Trypanosoma, and Trichomonas. This phenomenon has not been observed yet in Entamoeba histolytica. This work analyzed the in vitro effect of sodium nitroprusside, sodium nitrite and sodium nitrate (NOs) on E. histolytica apoptosis. Paras...
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Published in | Experimental parasitology Vol. 116; no. 3; pp. 257 - 265 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
San Diego, CA
Elsevier Inc
01.07.2007
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | Apoptosis has been described in some parasites like
Leishmania,
Trypanosoma, and
Trichomonas. This phenomenon has not been observed yet in
Entamoeba histolytica. This work analyzed the
in vitro effect of sodium nitroprusside, sodium nitrite and sodium nitrate (NOs) on
E. histolytica apoptosis. Parasites incubated for 1
h with NOs revealed apoptosis 6
h later (95% viability), demonstrated by YOPRO-1, TUNEL, DNA fragmentation and low ATP levels. The caspase inhibitor Z-VAD-FMK inhibited total intracellular cysteine protease activity (CPA) but had no effect on apoptosis. When treated with NOs some amebic functions like complement resistance and hemolytic activity decreased but CPA and erythrophagocytosis remained unchanged. After treatment
in vitro with NOs, parasite death was almost complete at 24
h; but when injected into hamster livers they disappeared in less than 6
h. These results show that apoptosis is induced
in vitro by NOs in
E. histolytica and renders them incapable of surviving in hamster’s livers. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0014-4894 1090-2449 |
DOI: | 10.1016/j.exppara.2007.01.011 |