Saccharomyces boulardii exerts renoprotection by modulating oxidative stress, renin angiotensin system and uropathogenic microbiota in a murine model of diabetes

• Published in: Life sciences (1973) Vol. 301; p. 120616
• Main Authors: Abreu, Isabel Cristina Mallostro Emerich de, Albuquerque, Raquel Cristina Melo Ferreira de, Brandão, Ana Beatriz Pereira, Barssotti, Leticia, de Souza, Lívia Bruni, Ferreira, Fabiana Gomes, Oliveira, Lilian Caroline Gonçalves de, Yokota, Rodrigo, Sparvoli, Luiz Gustavo, Dias, Danielle da Silva, Salgado, Miguel Angel Castillo, Taddei, Carla, De Angelis, Kátia, Casarini, Dulce Elena, Cunha, Tatiana Sousa
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 15.07.2022; Elsevier BV
• Subjects: ACE2; Albuminuria; Angiotensin; Angiotensin I; Angiotensin-converting enzyme 2; Animal models; Animals; Antioxidants; Biomarkers; Blood; Diabetes; Diabetes mellitus; Diabetic nephropathy; Dopamine; Fungi; Glucose; Intestinal microflora; Intestine; Microbiota; Modulation; Nephropathy; Oxidative stress; Peptides; Peptidyl-dipeptidase A; Probiotics; Renal function; Renin; Renin-angiotensin system; Saccharomyces boulardii; Serotonin; Streptozocin; Streptozotocin-diabetic mice; Structure-function relationships; Uropathogenic microbiota; Oxidative stress; Diabetic nephropathy; Albuminuria; Renin-angiotensin system; Uropathogenic microbiota; Streptozotocin-diabetic mice
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2022.120616

We aimed to investigate whether Saccharomyces boulardii strain might exert renoprotective effects by modulating renal renin angiotensin system, oxidative stress and intestinal microbiota in streptozotocin-diabetic mice. Thirty-six C57BL/6 male mice were divided into four groups: control (C), control + probiotic (CP), diabetes (D), diabetes + probiotic (DP). Diabetes was induced by one intraperitoneal injection of streptozotocin and Saccharomyces boulardii was administered by oral gavage for 8 weeks. Blood glucose, albuminuria and urinary volume were measured. Renal levels of angiotensin peptides (angiotensin I, II and 1–7) and the activities of angiotensin-converting enzyme (ACE) and ACE2 were determined, besides that, renal morphology, serotonin and dopamine levels and also microbiota composition were analyzed. Probiotics significantly increased C-peptide secretion and reduced blood glucose of diabetic animals. Saccharomyces boulardii also improved renal antioxidant defense, restored serotonin and dopamine concentration, and activated the renin-angiotensin system (RAS) vasodilator and antifibrotic axis. The modulation of these markers was associated with a beneficial impact on glomerular structure and renal function of diabetic treated animals. The phenotypic changes induced by Saccharomyces boulardii were also related to modulation of intestinal microbiota, evidenced by the decreased abundance of Proteus and Escherichia-Shigella, considered diabetic nephropathy biomarkers. Therefore, probiotic administration to streptozotocin-induced diabetic mice improves kidney structure and function in a murine model and might represent a reasonable strategy to counteract nephropathy-associated maladaptive responses in diabetes. Schematic representation showing the effects of diabetes and Saccharomyces boulardii on intrarenal molecules. The red arrows indicate the effects induced by diabetes, and the green arrows indicate the effects of Saccharomyces boulardi, on diabetic treated group. [Display omitted]