Development of the Mammalian Expression Vector System that can be Induced by IPTG and/or Lactose

• Published in: Journal of microbiology and biotechnology Vol. 30; no. 8; pp. 1124 - 1131
• Main Authors: Myung, Seung-Hyun, Park, Junghee, Han, Ji-Hye, Kim, Tae-Hyoung
• Format: Journal Article
• Language: English
• Published: Korea (South) The Korean Society for Microbiology and Biotechnology 28.08.2020; 한국미생물·생명공학회
• Subjects: Animals; Gene Expression Regulation - drug effects; HEK293 Cells; HeLa Cells; Humans; Isopropyl Thiogalactoside - pharmacology; Lac Operon - drug effects; Lactose - pharmacology; Research article; Transfection; 생물학; Mammalian inducible plasmid; Inducible vector; IPTG-responsive promoter
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.2003.03030

Techniques used for the regulation of gene expression facilitate studies of gene function and treatment of diseases via gene therapy. Many tools have been developed for the regulation of gene expression in mammalian cells. The Lac operon system induced with isopropyl β-D-1- thiogalactopyranoside (IPTG) is one of the employed inducible systems. IPTG mimics the molecular structure of allolactose and has a strong affinity for the corresponding repressor. IPTG is known to rapidly penetrate into mammalian cells and exhibits low toxicity. In the present study, we developed a new inducible expression system that could regulate the expression of genes in mammalian cells using IPTG. Here we confirm that unlike other vector systems based on the Lac operon, this expression system allows regulation of gene expression with lactose in the mammalian cells upon transfection. The co-treatment with IPTG and lactose could improve the regulatory efficiency of the specific target gene expression. The regulation of gene expression with lactose has several benefits. Lactose is safe in humans as compared to other chemical substances and is easily available, making this technique very cost-effective.