Intracellular substrates of a heme-containing ascorbate oxidase in Pleurotus ostreatus

• Published in: The journal of microbiology Vol. 47; no. 2; pp. 178 - 186
• Main Authors: Lee, S.R. (Chonnam National University Medical School, Gwangju, Republic of Korea), E-mail: leesr@chonnam.ac.kr, Joo, W.J. (Seoul National University, Seoul, Republic of Korea), Baek, Y.U. (Seoul National University, Seoul, Republic of Korea), Lee, Y.K. (Seoul National University, Seoul, Republic of Korea), Yu, S.W. (Seoul National University, Seoul, Republic of Korea), Kim, Y.R. (Seoul National University, Seoul, Republic of Korea), Chay, K.O. (Chonnam National University Medical School, Gwangju, Republic of Korea), Cho, S.H. (Seoul National University, Seoul, Republic of Korea), Kang, S.O. (Seoul National University, Seoul, Republic of Korea), E-mail: kangsaou@snu.ac.kr
• Format: Journal Article
• Language: English
• Published: Heidelberg The Microbiological Society of Korea 01.04.2009; Springer Nature B.V; 한국미생물학회
• Subjects: Ascorbate Oxidase - chemistry; Ascorbate Oxidase - metabolism; Biomedical and Life Sciences; Copper; Enzymes; Free radicals; Fungal Proteins - chemistry; Fungal Proteins - metabolism; Heme - metabolism; Hydrogen peroxide; Life Sciences; Microbiology; Oxidation; Oxidation-Reduction; OXIDOREDUCTASES; OXIDORREDUCTASAS; OXYDOREDUCTASE; Pleurotus - chemistry; Pleurotus - enzymology; PLEUROTUS OSTREATUS; Substrate Specificity; Substrates; Trace elements; 생물학; South Korea; substrates; ascorbate oxidase
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-008-0307-8

A novel heme-containing ascorbate oxidase isolated from oyster mushroom, Pleurotus ostreatus, catalyzes oxidation of ascorbic acid (Kim et al., 1996). In this report, we describe the identification of intracellular substrates of the enzyme in the mushroom. Six compounds, which can serve as substrate of the heme-containing ascorbate oxidase, were identified as L-ascorbic acid, D-erythroascorbic acid, 5-O-(α-D-glucopyranosyl)-D-erythroascorbic acid, 5-O-(α-D-xylopyranosyl)-D-erythroascorbic acid, 5-methyl-5-O-(α-D-glucopyranosyl)-D-erythroascorbic acid, and 5-methyl-5-O-(α-D-xylopyranosyl)-D-erythroascorbic acid. All of the compounds were oxidized at a significant rate by the heme-containing ascorbate oxidase. Oxidation of the compounds produced equimolar amounts of hydrogen peroxide per mole of substrate.