Biokinetics of protein degrading Clostridium cadaveris and Clostridium sporogenes in batch and continuous mode of operations

• Published in: Journal of microbiology and biotechnology Vol. 30; no. 4; pp. 533 - 539
• Main Authors: Koo, Taewoan, Jannat, Md Abu Hanifa, Hwang, Seokhwan
• Format: Journal Article
• Language: English
• Published: Korea (South) The Korean Society for Microbiology and Biotechnology 28.04.2020; 한국미생물·생명공학회
• Subjects: Batch Cell Culture Techniques; Biodegradation, Environmental; Bioreactors - microbiology; Clostridium - growth & development; Clostridium - metabolism; Kinetics; Peptones - metabolism; Proteins - metabolism; Research article; RNA, Ribosomal, 16S - genetics; 생물학; Clostridium sporogenes; Clostridium cadaveris; Protein degradation; quantitative real-time PCR; biokinetic analysis
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/JMB.1908.08054

A quantitative real-time polymerase chain reaction (QPCR) was applied to estimate biokinetic coefficients of and , which utilize protein as carbon source. Experimental data of changes in peptone concentration and 16S rRNA gene copy numbers of and were fitted to model. The fourth-order Runge-Kutta approximation with non-linear least squared analysis was employed to solve the ordinary differential equations to estimate biokinetic coefficients. The maximum specific growth rate ( ), half saturation concentration ( ), growth yield ( ), and decay coefficient ( ) of and were 0.73 ± 0.05 and 1.35 ± 0.32 h , 6.07 ± 1.52 and 5.67 ± 1.53 g/L, 2.25 ± 0.75 × 10 and 7.92 ± 3.71 × 10 copies/g, 0.002 ± 0.003 and 0.002 ± 0.001 h , respectively. The theoretical specific growth rate of always exceeded than that of at peptone concentration higher than 3.62 g/L. When the influent peptone concentration was 5.0 g/L, the concentration of C.cadaveris gradually decreased to the steady value of 2.9 × 10 copies/mL at 4 hours HRT, which indicates 67.1% of the initial population reduction, but the wash out occurred at 1.9 and 3.2 hours HRTs. The 16S rRNA gene copy numbers of gradually decreased to steady values ranging from 1.1 × 10 to 2.9 × 10 copies/mL. species was predicted to wash out at an HRT of 1.6 h.