Biokinetics of protein degrading Clostridium cadaveris and Clostridium sporogenes in batch and continuous mode of operations
A quantitative real-time polymerase chain reaction (QPCR) was applied to estimate biokinetic coefficients of and , which utilize protein as carbon source. Experimental data of changes in peptone concentration and 16S rRNA gene copy numbers of and were fitted to model. The fourth-order Runge-Kutta ap...
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Published in | Journal of microbiology and biotechnology Vol. 30; no. 4; pp. 533 - 539 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Korea (South)
The Korean Society for Microbiology and Biotechnology
28.04.2020
한국미생물·생명공학회 |
Subjects | |
Online Access | Get full text |
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Summary: | A quantitative real-time polymerase chain reaction (QPCR) was applied to estimate biokinetic coefficients of
and
, which utilize protein as carbon source. Experimental data of changes in peptone concentration and 16S rRNA gene copy numbers of
and
were fitted to model. The fourth-order Runge-Kutta approximation with non-linear least squared analysis was employed to solve the ordinary differential equations to estimate biokinetic coefficients. The maximum specific growth rate (
), half saturation concentration (
), growth yield (
), and decay coefficient (
) of
and
were 0.73 ± 0.05 and 1.35 ± 0.32 h
, 6.07 ± 1.52 and 5.67 ± 1.53 g/L, 2.25 ± 0.75 × 10
and 7.92 ± 3.71 × 10
copies/g, 0.002 ± 0.003 and 0.002 ± 0.001 h
, respectively. The theoretical specific growth rate of
always exceeded than that of
at peptone concentration higher than 3.62 g/L. When the influent peptone concentration was 5.0 g/L, the concentration of C.cadaveris gradually decreased to the steady value of 2.9 × 10
copies/mL at 4 hours HRT, which indicates 67.1% of the initial population reduction, but the wash out occurred at 1.9 and 3.2 hours HRTs. The 16S rRNA gene copy numbers of
gradually decreased to steady values ranging from 1.1 × 10
to 2.9 × 10
copies/mL.
species was predicted to wash out at an HRT of 1.6 h. |
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ISSN: | 1017-7825 1738-8872 |
DOI: | 10.4014/JMB.1908.08054 |