Development of serological procedures for rapid and reliable detection of wheat streak mosaic virus in a single wheat curl mite
Wheat streak mosaic virus (WSMV) is transmitted by the wheel curl mite (WCM), Aceria tosichella. Immunofluorescent and dot-immunobinding assays were developed to detect the presence of WSMV in single WCM. Virus-specific immunofluorescent microscopy detected WSMV near the anterior end of viruliferous...
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Published in | Plant disease Vol. 81; no. 3; p. 250 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
01.03.1997
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Abstract | Wheat streak mosaic virus (WSMV) is transmitted by the wheel curl mite (WCM), Aceria tosichella. Immunofluorescent and dot-immunobinding assays were developed to detect the presence of WSMV in single WCM. Virus-specific immunofluorescent microscopy detected WSMV near the anterior end of viruliferous WCM. With dot-immunobinding assay, WSMV was detected in WCM fed on WSMV-infected wheat (Triticum aestivum) but not in WCM maintained on healthy plants. Both immunofluorescent and dot-immunobinding assays were sufficiently sensitive to detect WSMV in individual WCM, providing a means to determine the percentage of viruliferous WCM in field collections |
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AbstractList | Wheat streak mosaic virus (WSMV) is transmitted by the wheat curl mite (WCM), Aceria tosichella. Immunofluorescent and dot-immunobinding assays were developed to detect the presence of WSMV in single WCM. Virus-specific immunofluorescent microscopy detected WSMV near the anterior end of viruliferous WCM. With dot-immunobinding assay, WSMV was detected in WCM fed on WSMV-infected wheat (Triticum aestivum) but not in WCM maintained on healthy plants. Both immunofluorescent and dot-immunobinding assays were sufficiently sensitive to detect WSMV in individual WCM, providing a means to determine the percentage of viruliferous WCM in field collections. Wheat streak mosaic virus (WSMV) is transmitted by the wheel curl mite (WCM), Aceria tosichella. Immunofluorescent and dot-immunobinding assays were developed to detect the presence of WSMV in single WCM. Virus-specific immunofluorescent microscopy detected WSMV near the anterior end of viruliferous WCM. With dot-immunobinding assay, WSMV was detected in WCM fed on WSMV-infected wheat (Triticum aestivum) but not in WCM maintained on healthy plants. Both immunofluorescent and dot-immunobinding assays were sufficiently sensitive to detect WSMV in individual WCM, providing a means to determine the percentage of viruliferous WCM in field collections |
Author | Mahmood, T. (University of Nebraska, Scottsbluff.) French, R.C Hein, G.L |
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Snippet | Wheat streak mosaic virus (WSMV) is transmitted by the wheel curl mite (WCM), Aceria tosichella. Immunofluorescent and dot-immunobinding assays were developed... Wheat streak mosaic virus (WSMV) is transmitted by the wheat curl mite (WCM), Aceria tosichella. Immunofluorescent and dot-immunobinding assays were developed... |
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Title | Development of serological procedures for rapid and reliable detection of wheat streak mosaic virus in a single wheat curl mite |
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