Development of serological procedures for rapid and reliable detection of wheat streak mosaic virus in a single wheat curl mite

Wheat streak mosaic virus (WSMV) is transmitted by the wheel curl mite (WCM), Aceria tosichella. Immunofluorescent and dot-immunobinding assays were developed to detect the presence of WSMV in single WCM. Virus-specific immunofluorescent microscopy detected WSMV near the anterior end of viruliferous...

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Bibliographic Details
Published inPlant disease Vol. 81; no. 3; p. 250
Main Authors Mahmood, T. (University of Nebraska, Scottsbluff.), Hein, G.L, French, R.C
Format Journal Article
LanguageEnglish
Published United States 01.03.1997
Subjects
EXPERIMENTACION
EXPERIMENTATION
IMMUNOFLUORESCENCE
INMUNOFLUORESCENCIA
MICROSCOPIA
MICROSCOPIE
TECHNIQUE ANALYTIQUE
TECHNIQUE IMMUNOENZYMATIQUE
TECNICAS ANALITICAS
TECNICAS INMUNOENZIMATICAS
TRITICUM AESTIVUM
VECTEUR DE MALADIE
VECTORES
VIRUS DE LAS PLANTAS
VIRUS DES VEGETAUX
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Summary:Wheat streak mosaic virus (WSMV) is transmitted by the wheel curl mite (WCM), Aceria tosichella. Immunofluorescent and dot-immunobinding assays were developed to detect the presence of WSMV in single WCM. Virus-specific immunofluorescent microscopy detected WSMV near the anterior end of viruliferous WCM. With dot-immunobinding assay, WSMV was detected in WCM fed on WSMV-infected wheat (Triticum aestivum) but not in WCM maintained on healthy plants. Both immunofluorescent and dot-immunobinding assays were sufficiently sensitive to detect WSMV in individual WCM, providing a means to determine the percentage of viruliferous WCM in field collections
Bibliography:H20
9732764
ISSN:0191-2917
1943-7692
DOI:10.1094/PDIS.1997.81.3.250