Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake
is an ectomycorrhizal fungus, related with the host of . Most of studies on have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and immune activity of this strain. is known for its unique fragrance in Eastern Asia. The most major component of its scent is (R)-(-)-1-octen-3...
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| Published in | Journal of microbiology and biotechnology Vol. 30; no. 2; pp. 296 - 305 |
|---|---|
| Main Authors | , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Korea (South)
Korean Society for Microbiology and Biotechnology
28.02.2020
한국미생물·생명공학회 |
| Subjects | |
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| Abstract | is an ectomycorrhizal fungus, related with the host of
. Most of studies on
have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and immune activity of this strain.
is known for its unique fragrance in Eastern Asia. The most major component of its scent is (R)-(-)-1-octen-3-ol and is biosynthesized from the substrate linoleic acid by the sequential reaction of lipoxygenase and peroxide lyase. Here, we report for the first time the biosynthesis of (R)-(-)- 1-octen-3-ol of
using the yeast
as a host. In this study, cDNA genes correlated with these reactions were cloned from
, and expression studies of theses genes were carried out in the yeast
. The product of these genes expression study was carried out with Western blotting. The biosynthesis of (R)-(-)- 1-octen-3-ol of
in recombinant
was subsequently identified with GC-MS chromatography analysis. The biosynthesis of (R)-(-)-1-octen-3-ol with
represents a significant step forward. |
|---|---|
| AbstractList | is an ectomycorrhizal fungus, related with the host of
. Most of studies on
have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and immune activity of this strain.
is known for its unique fragrance in Eastern Asia. The most major component of its scent is (R)-(-)-1-octen-3-ol and is biosynthesized from the substrate linoleic acid by the sequential reaction of lipoxygenase and peroxide lyase. Here, we report for the first time the biosynthesis of (R)-(-)- 1-octen-3-ol of
using the yeast
as a host. In this study, cDNA genes correlated with these reactions were cloned from
, and expression studies of theses genes were carried out in the yeast
. The product of these genes expression study was carried out with Western blotting. The biosynthesis of (R)-(-)- 1-octen-3-ol of
in recombinant
was subsequently identified with GC-MS chromatography analysis. The biosynthesis of (R)-(-)-1-octen-3-ol with
represents a significant step forward. Tricholoma matsutake is an ectomycorrhizal fungus, related with the host of Pinus densiflora. Most of studies on T. matsutake have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and immune activity of this strain. T. matsutake is known for its unique fragrance in Eastern Asia. The most major component of its scent is (R)-(-)-1-octen-3-ol and is biosynthesized from the substrate linoleic acid by the sequential reaction of lipoxygenase and peroxide lyase. Here, we report for the first time the biosynthesis of (R)-(-)- 1-octen-3-ol of T. matsutake using the yeast Saccharomyces cerevisiae as a host. In this study, cDNA genes correlated with these reactions were cloned from T. matsutake, and expression studies of theses genes were carried out in the yeast Saccharomyces cerevisiae. The product of these genes expression study was carried out with Western blotting. The biosynthesis of (R)-(-)- 1-octen-3-ol of T. matsutake in recombinant Saccharomyces cerevisiae was subsequently identified with GC-MS chromatography analysis. The biosynthesis of (R)-(-)-1-octen-3-ol with S. cerevisiae represents a significant step forward. KCI Citation Count: 0 Tricholoma matsutake is an ectomycorrhizal fungus, related with the host of Pinus densiflora . Most of studies on T. matsutake have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and immune activity of this strain. T. matsutake is known for its unique fragrance in Eastern Asia. The most major component of its scent is ( R )-(-)-1-octen-3-ol and is biosynthesized from the substrate linoleic acid by the sequential reaction of lipoxygenase and peroxide lyase. Here, we report for the first time the biosynthesis of ( R )-(-)- 1-octen-3-ol of T. matsutake using the yeast Saccharomyces cerevisiae as a host. In this study, cDNA genes correlated with these reactions were cloned from T. matsutake , and expression studies of theses genes were carried out in the yeast Saccharomyces cerevisiae . The product of these genes expression study was carried out with Western blotting. The biosynthesis of ( R )-(-)- 1-octen-3-ol of T. matsutake in recombinant Saccharomyces cerevisiae was subsequently identified with GC-MS chromatography analysis. The biosynthesis of ( R )-(-)-1-octen-3-ol with S. cerevisiae represents a significant step forward. |
| Author | Kwon, Hae-Jun Kim, Mi-Gyeong Kim, Young-Guk Arioka, Manabu di Luccio, Eric Kim, Dong-Hyun Choi, Doo-Ho Yoon, Hyeok-Jun Jeong, Min-Ji Kim, Jong-Guk Lee, Nan-Yeong |
| AuthorAffiliation | 2 School of Life Science, College of Natural Sciences, Kyungpook National University, Daegu 41566, Republic of Korea 3 Department of Biotechnology, The University of Tokyo 1-1-1 Yayoi, Bunkyo-ku Tokyo 11-8657 Japan 1 School of Life Science and Biotechnology, Kyungpook National University, Daegu 4566, Republic of Korea |
| AuthorAffiliation_xml | – name: 2 School of Life Science, College of Natural Sciences, Kyungpook National University, Daegu 41566, Republic of Korea – name: 3 Department of Biotechnology, The University of Tokyo 1-1-1 Yayoi, Bunkyo-ku Tokyo 11-8657 Japan – name: 1 School of Life Science and Biotechnology, Kyungpook National University, Daegu 4566, Republic of Korea |
| Author_xml | – sequence: 1 givenname: Nan-Yeong surname: Lee fullname: Lee, Nan-Yeong organization: School of Life Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea – sequence: 2 givenname: Doo-Ho surname: Choi fullname: Choi, Doo-Ho organization: School of Life Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea – sequence: 3 givenname: Mi-Gyeong surname: Kim fullname: Kim, Mi-Gyeong organization: School of Life Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea – sequence: 4 givenname: Min-Ji surname: Jeong fullname: Jeong, Min-Ji organization: School of Life Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea – sequence: 5 givenname: Hae-Jun surname: Kwon fullname: Kwon, Hae-Jun organization: School of Life Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea – sequence: 6 givenname: Dong-Hyun surname: Kim fullname: Kim, Dong-Hyun organization: School of Life Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea – sequence: 7 givenname: Young-Guk surname: Kim fullname: Kim, Young-Guk organization: School of Life Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea – sequence: 8 givenname: Eric surname: di Luccio fullname: di Luccio, Eric organization: School of Life Science, College of Natural Sciences, Kyungpook National University, Daegu 41566, Republic of Korea – sequence: 9 givenname: Manabu surname: Arioka fullname: Arioka, Manabu organization: Department of Biotechnology, The University of Tokyo 1-1-1 Yayoi, Bunkyo-ku Tokyo 113-8657 Japan – sequence: 10 givenname: Hyeok-Jun surname: Yoon fullname: Yoon, Hyeok-Jun organization: School of Life Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea – sequence: 11 givenname: Jong-Guk surname: Kim fullname: Kim, Jong-Guk organization: School of Life Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea |
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| Copyright | Copyright©2020 by The Korean Society for Microbiology and Biotechnology 2020 |
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| Keywords | lipoxygenase (R)-(-)-1-octen-3-ol Tricholoma matsutake Saccharomyces cerevisiae hydroperoxide lyase |
| Language | English |
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| Snippet | is an ectomycorrhizal fungus, related with the host of
. Most of studies on
have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and... Tricholoma matsutake is an ectomycorrhizal fungus, related with the host of Pinus densiflora . Most of studies on T. matsutake have focused on mycelial growth,... Tricholoma matsutake is an ectomycorrhizal fungus, related with the host of Pinus densiflora. Most of studies on T. matsutake have focused on mycelial growth,... |
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| SubjectTerms | Aldehyde-Lyases - genetics Cloning, Molecular Cytochrome P-450 Enzyme System - genetics Fermentation Gene Expression Isoenzymes Lipoxygenase - genetics Octanols - metabolism Recombinant Proteins Research article Saccharomyces cerevisiae - metabolism Temperature Transformation, Genetic Tricholoma - enzymology Tricholoma - genetics 생물학 |
| Title | Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake |
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