Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake

is an ectomycorrhizal fungus, related with the host of . Most of studies on have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and immune activity of this strain. is known for its unique fragrance in Eastern Asia. The most major component of its scent is (R)-(-)-1-octen-3...

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Published inJournal of microbiology and biotechnology Vol. 30; no. 2; pp. 296 - 305
Main Authors Lee, Nan-Yeong, Choi, Doo-Ho, Kim, Mi-Gyeong, Jeong, Min-Ji, Kwon, Hae-Jun, Kim, Dong-Hyun, Kim, Young-Guk, di Luccio, Eric, Arioka, Manabu, Yoon, Hyeok-Jun, Kim, Jong-Guk
Format Journal Article
LanguageEnglish
Published Korea (South) Korean Society for Microbiology and Biotechnology 28.02.2020
한국미생물·생명공학회
Subjects
Aldehyde-Lyases - genetics
Cloning, Molecular
Cytochrome P-450 Enzyme System - genetics
Fermentation
Gene Expression
Isoenzymes
Lipoxygenase - genetics
Octanols - metabolism
Recombinant Proteins
Research article
Saccharomyces cerevisiae - metabolism
Temperature
Transformation, Genetic
Tricholoma - enzymology
Tricholoma - genetics
생물학
lipoxygenase
(R)-(-)-1-octen-3-ol
Tricholoma matsutake
Saccharomyces cerevisiae
hydroperoxide lyase
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Summary:is an ectomycorrhizal fungus, related with the host of . Most of studies on have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and immune activity of this strain. is known for its unique fragrance in Eastern Asia. The most major component of its scent is (R)-(-)-1-octen-3-ol and is biosynthesized from the substrate linoleic acid by the sequential reaction of lipoxygenase and peroxide lyase. Here, we report for the first time the biosynthesis of (R)-(-)- 1-octen-3-ol of using the yeast as a host. In this study, cDNA genes correlated with these reactions were cloned from , and expression studies of theses genes were carried out in the yeast . The product of these genes expression study was carried out with Western blotting. The biosynthesis of (R)-(-)- 1-octen-3-ol of in recombinant was subsequently identified with GC-MS chromatography analysis. The biosynthesis of (R)-(-)-1-octen-3-ol with represents a significant step forward.
Bibliography:These authors contributed equally to this work.
ISSN:1017-7825
1738-8872
DOI:10.4014/jmb.2001.01049