Standardization of large scale production of homologous live attenuated PPR vaccine in India

• Published in: Tropical animal health and production Vol. 40; no. 1; pp. 11 - 16
• Main Authors: Hegde, Raveendra, Gomes, Amitha R, Byregowda, S. M, Hugar, Paramananda, Giridhar, P, Renukaprasad, C
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 2008; Springer Netherlands; Springer Nature B.V
• Subjects: Animals; Biomedical and Life Sciences; Cercopithecus aethiops; Goats; Guinea Pigs; Homologous vaccine; Life Sciences; Live attenuated; Mice; Moisture content; Multiplicity of infection; Original Paper; peste des petits ruminants; Peste-des-petits-ruminants virus - immunology; Quality control; Quality control tests; Ruminantia; Sheep; TCID; Vaccine production; Vaccines; Vaccines, Attenuated - chemistry; Vaccines, Attenuated - immunology; Vero Cells; Veterinary Medicine/Veterinary Science; Viral Vaccines - biosynthesis; Viral Vaccines - chemistry; Viral Vaccines - immunology; Zoology; India; Multiplicity of infection; Live attenuated; Homologous vaccine; Vaccine production; TCID; Vero cells; India; Quality control tests
• ISSN: 0049-4747; 1573-7438
• DOI: 10.1007/s11250-007-9039-8

Live attenuated homologous vaccine against peste des petits ruminants of sheep and goats was produced on a large scale basis in roller culture bottles using seed virus developed at the Indian Veterinary Research Institute, Muktheswar, India. Vero cells between 130-150 passages with six percent foetal calf serum were used for the production of vaccine. The cells were infected with 0.01 multiplicity of infection and harvested when the cytopathic effect was 80%. The vaccine was freezedried in order to maintain the stability of the vaccine. Identity test and titration was performed and the vaccine titre was monitored to be minimum of 10⁵/100 doses. In-house sterility tests and quality control tests using experimental animals and small ruminants were performed. The vacuum and moisture content of the vaccine were also regulated to be within the normal limits.