Transcriptomic Approach for Understanding the Adaptation of Salmonella enterica to Contaminated Produce

• Published in: Journal of microbiology and biotechnology Vol. 30; no. 11; pp. 1729 - 1738
• Main Authors: Park, Sojung, Nam, Eun Woo, Kim, Yeeun, Lee, Seohyeon, Kim, Seul I, Yoon, Hyunjin
• Format: Journal Article
• Language: English
• Published: Korea (South) Korean Society for Microbiology and Biotechnology 28.11.2020; 한국미생물·생명공학회
• Subjects: Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Food Contamination; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Genomic Islands - genetics; Life Style; Raphanus - microbiology; Research article; Salmonella enterica - genetics; Salmonella enterica - metabolism; Salmonella Infections; Sequence Analysis, RNA; Transcriptome; Virulence - genetics; 생물학; transcriptomics; Salmonella enterica; epiphytic; produce; planktonic
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/JMB.2007.07036

Salmonellosis is a form of gastroenteritis caused by infection. The main transmission route of salmonellosis has been identified as poorly cooked meat and poultry products contaminated with . However, in recent years, the number of outbreaks attributed to contaminated raw produce has increased dramatically. To understand how adapts to produce, transcriptomic analysis was conducted on enterica serovar Virchow exposed to fresh-cut radish greens. Considering the different lifestyles in contact with fresh produce, such as motile and sessile lifestyles, total RNA was extracted from planktonic and epiphytic cells separately. Transcriptomic analysis of S. Virchow cells revealed different transcription profiles between lifestyles. During bacterial adaptation to fresh-cut radish greens, planktonic cells were likely to shift toward anaerobic metabolism, exploiting nitrate as an electron acceptor of anaerobic respiration, and utilizing cobalamin as a cofactor for coupled metabolic pathways. Meanwhile, cells adhering to plant surfaces showed coordinated upregulation in genes associated with translation and ribosomal biogenesis, indicating dramatic cellular reprogramming in response to environmental changes. In accordance with the extensive translational response, epiphytic cells showed an increase in the transcription of genes that are important for bacterial motility, nucleotide transporter/metabolism, cell envelope biogenesis, and defense mechanisms. Intriguingly, pathogenicity island (SPI)-1 and SPI-2 displayed up- and downregulation, respectively, regardless of lifestyles in contact with the radish greens, suggesting altered virulence during adaptation to plant environments. This study provides molecular insights into adaptation to plants as an alternative environmental reservoir.