Identification of genes involved in phosphate solubilization and drought stress tolerance in chickpea symbiont Mesorhizobium ciceri Ca181

• Published in: Archives of microbiology Vol. 203; no. 3; pp. 1167 - 1174
• Main Authors: Yadav, Akhilesh, Singh, Raghvendra Pratap, Singh, Asha Lata, Singh, Major
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.04.2021; Springer Nature B.V
• Subjects: Bacteria; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cell Biology; Chickpeas; Cicer - microbiology; Defective mutant; Drought; Droughts; Ecology; Gene sequencing; Genes; Genes, Bacterial - genetics; Genomes; Gluconic acid; Life Sciences; Mesorhizobium; Mesorhizobium - genetics; Microbial Ecology; Microbiology; Molecular modelling; Nitrogen Fixation; Nitrogenation; Nodules; Original Paper; Phosphates - metabolism; Phosphorus; Plant growth; Plant roots; Plant Roots - microbiology; Probiotics; Root nodules; Solubilization; Stress, Physiological - genetics; Symbiosis; Ca181; Chickpea; Phosphate solubilization; Drought; Symbiont; Mutant; Mesorhizobium ciceri Ca181
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-020-02109-1

Chickpea plant root colonizing bacteria Mesorhizobium ciceri Ca181 promotes plant growth and development through symbiotic association with root nodules. The potentially beneficial effects on plants generated due to this bacterium are mineral nutrient solubilization, abiotic stress tolerance, and nitrogen-fixation, though the molecular mechanisms underlying these probiotic capacities are still largely unknown. Hence, this study aims to describe the molecular mechanism of M. ciceri Ca181 in drought stress tolerance and phosphorus solubilization. Here we have developed the transposon inserted mutant library of strain Ca181 and further screened it to identify the phosphorous solubilization and PEG-induced drought stress tolerance defective mutants, respectively. Resultantly, a total of four and three mutants for phosphorous solubilization and drought stress tolerance were screened and identified. Consequently, Southern blot confirmation was done for the cross verification of insertions and stability in the genome. Through the sequencing of each mutant, the interrupted gene was confirmed, and the finding revealed that the production of gluconic acid is necessary for phosphorus solubilization, while  otsA , Auc, and Usp genes were involved in the mechanism of drought stress tolerance in M. ciceri Ca181.