Autogenous modulation of the Bacillus subtilis sacB-levB-yveA levansucrase operon by the levB transcript

• Published in: Microbiology (Society for General Microbiology) Vol. 150; no. 11; pp. 3669 - 3679
• Main Authors: Daguer, Jean-Pierre, Geissmann, Thomas, Petit-Glatron, Marie-Francoise, Chambert, Regis
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.11.2004; Society for General Microbiology
• Subjects: Amino Acid Transport Systems, Acidic - biosynthesis; Amino Acid Transport Systems, Acidic - genetics; Bacillus subtilis; Bacillus subtilis - enzymology; Bacillus subtilis - genetics; Bacterial Proteins - metabolism; Bacteriology; Base Sequence; Biological and medical sciences; Disaccharides - metabolism; Fructans - metabolism; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Bacterial; Gene Silencing; Glycoside Hydrolases - genetics; Glycoside Hydrolases - metabolism; Hexosyltransferases - biosynthesis; Hexosyltransferases - genetics; Hexosyltransferases - metabolism; Microbiology; Miscellaneous; Molecular Sequence Data; Nucleic Acid Conformation; Operon; RNA, Bacterial - analysis; RNA, Bacterial - metabolism; RNA, Messenger - analysis; RNA, Messenger - metabolism; RNA-Binding Proteins - metabolism; Sucrose - metabolism; Transcription Factors - metabolism; Transcription, Genetic; Sucrose; Enzyme; Bacillus subtilis; Levansucrase; Operon; Transferases; Glycosyltransferases; Bacillaceae; Protein; Transcription termination; Messenger RNA; Bacillales; Gene; Bacteria; Hexosyltransferases; Display
• ISSN: 1350-0872; 1465-2080
• DOI: 10.1099/mic.0.27366-0

Institut Jacques Monod, Laboratoire Génétique et Membranes, CNRS – Universités Paris 6 et Paris 7, Tour 43, 2 place Jussieu, 75251 Paris Cedex 05, France Correspondence Régis Chambert chambert{at}ccr.jussieu.fr Silencing of levB , the second structural gene of the tricistronic levansucrase operon encoding the endolevanase LevB, decreases the level of levansucrase expression in Bacillus subtilis . Conversely, independent expression of levB greatly stimulates operon expression. This autogenous effect is mediated by the levB transcript, which carries an internal sequence (5'-AAAGCAGGCAA-3') involved in the enhancing effect. In vitro , the levB transcript displays an affinity for the N-terminal fragment of SacY ( K D 0·2 µM), the regulatory protein that prevents transcription termination of the levansucrase operon. This positive-feedback loop leads to an increase in the operon expression when B. subtilis is growing in the presence of high sucrose concentrations. Under these conditions, extracellular levan synthesized by the fructosyl polymerase activity of levansucrase can be degraded mainly into levanbiose by the action of LevB. Levanbiose is neither taken up nor metabolized by the bacteria. This work modifies the present view of the status of levansucrase in B. subtilis physiology. Abbreviations: RAT, ribonucleic anti-terminator