Tissue Engineering Based on Cell Sheet Technology

Cell sheet technology enables novel approaches to tissue engineering without the use of biodegradable scaffolds. Cell sheet technology consists of a temperature‐responsive culture dish, which enables reversible cell adhesion to and detachment from the dish surface by controllable hydrophobicity of t...

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Published inAdvanced materials (Weinheim) Vol. 19; no. 20; pp. 3089 - 3099
Main Authors Matsuda, N., Shimizu, T., Yamato, M., Okano, T.
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 19.10.2007
WILEY‐VCH Verlag
Subjects
Biocompatible materials
Bionanotechnology
Cells
Polymers
Tissue engineering
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Summary:Cell sheet technology enables novel approaches to tissue engineering without the use of biodegradable scaffolds. Cell sheet technology consists of a temperature‐responsive culture dish, which enables reversible cell adhesion to and detachment from the dish surface by controllable hydrophobicity of the surface. This allows for a non‐invasive harvest of cultured cells as an intact monolayer cell sheet including deposited extra cellular matrices. The monolayer cell sheet can be transplanted to host tissues without using biodegradable scaffolds and sutures. Thick tissue constructs and patterned cell sheets using two or more kinds of cell source are also developed by means of layered cell sheets in vitro. This Progress Report summarizes temperature‐controlled cell adhesion‐detachment behavior and applications of the cell sheet technology to regeneration of cornea, periodontal ligament, bladder epithelia, oesophageal epithelia, myocardium, and liver. Cell sheet technology utilizing of temperature‐responsive culture dishes has been applied to tissue engineering. Via this technology, cell sheets can be transplanted to host tissues without using biodegradable scaffolds. This Progress Report summarizes temperature‐controlled cell adhesion‐detachment behavior and applications of the cell sheet technology to regeneration of cornea, periodontal ligament, bladder epithelia, oesophageal epithelia, myocardium, and liver.
Bibliography:ArticleID:ADMA200701978
We are gratefully thankful to our colleagues, Prof. Isao Ishikawa, Mr. Hidekazu Sekine (Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University), and Dr. Umber Cheema (University College London) for their kind assistance. The present work was supported by grants for High-Tech Research Center Program, Center of Excellence (COE) Program for the 21st Century from Ministry of Education, Culture, Sports, Science and Technology, Japan.
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Ministry of Education, Culture, Sports, Science and Technology, Japan
istex:4A67A81822E49A6E0BC131B1FDDA4BA31C92FA62
We are gratefully thankful to our colleagues, Prof. Isao Ishikawa, Mr. Hidekazu Sekine (Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University), and Dr. Umber Cheema (University College London) for their kind assistance. The present work was supported by grants for High‐Tech Research Center Program, Center of Excellence (COE) Program for the 21st Century from Ministry of Education, Culture, Sports, Science and Technology, Japan.
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ISSN:0935-9648
1521-4095
DOI:10.1002/adma.200701978