Serum Autoantibodies in Mice Immunized with Syngeneic Testicular Germ Cells Alone

• Published in: American journal of reproductive immunology (1989) Vol. 70; no. 6; pp. 509 - 517
• Main Authors: Hirai, Shuichi, Naito, Munekazu, Terayama, Hayato, Hatayama, Naoyuki, Qu, Ning, Musha, Muhetaerjiang, Itoh, Masahiro
• Format: Journal Article
• Language: English
• Published: Denmark Blackwell Publishing Ltd 01.12.2013; Wiley Subscription Services, Inc
• Subjects: Animals; Autoantibodies; Autoantibodies - blood; Autoantibodies - immunology; Autoimmune Diseases - immunology; Autoimmune Diseases - pathology; Blotting, Western; Enzyme-Linked Immunosorbent Assay; epididymis; germ cells; Immunoassay; Immunohistochemistry; Male; Mice; Mice, Inbred Strains; mouse; Orchitis - immunology; Orchitis - pathology; Seminiferous Epithelium - immunology; Seminiferous Epithelium - pathology; spermatozoa; Spermatozoa - immunology; testis; Testis - cytology; mouse; Autoantibodies; testis; epididymis; spermatozoa; germ cells
• ISSN: 1046-7408; 1600-0897
• DOI: 10.1111/aji.12145

Problem The aim of this study was to identify the characteristics of serum autoantibodies in experimental autoimmune orchitis (EAO) induced by immunization with syngeneic testicular germ cells (TGC) alone in mice. Method of study The serum autoantibodies were analyzed by enzyme‐linked immunosorbent assay (ELISA), Western blotting, and immunohistochemical staining. Results Serum autoantibody titers against TGC and epididymal spermatozoa (ES) gradually increased with the development of EAO. At the initial stage of EAO, serum autoantibodies reacted with only a few antigens of TGC and ES, while in the later stage, the reactions with various antigens of TGC and ES occurred. However, the autoantibodies were specific to the testis and epididymis but not to other organs. Conclusion These results indicate that titers and kinds of autoantibodies in TGC‐immunized mice increase with EAO development and they are specific to TGC and ES.