HPV16 E6 variants: Frequency, association with HPV types and in silico analysis of the identified novel variants
High‐risk human papillomavirus (HPV) types, specifically HPV 16 E6 variants are involved in viral persistence and the development of cervical lesions. India contributes to 1/3rd of the global cervical cancer deaths; however, information on E6 variants in the Indian population is limited. Information...
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Published in | Journal of medical virology Vol. 86; no. 6; pp. 968 - 974 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Blackwell Publishing Ltd
01.06.2014
Wiley Subscription Services, Inc |
Subjects | |
Online Access | Get full text |
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Summary: | High‐risk human papillomavirus (HPV) types, specifically HPV 16 E6 variants are involved in viral persistence and the development of cervical lesions. India contributes to 1/3rd of the global cervical cancer deaths; however, information on E6 variants in the Indian population is limited. Information on these variants is essential for successful implementation of cervical cancer immunization programs. The E6 variants and their possible biological implications to the outcome of infection were studied in women attending the Tata Memorial Hospital, Mumbai, India. Cervical cancer patients with HPV 16 as a single infection (n = 33), co‐infection with another HPV type (n = 20) or with multiple types (n = 10) were examined for HPV16 E6 variants using PCR and sequence analysis. The variants were identified using the prototype sequence (HPV 16R) belonging to the European lineage. The results revealed that the European T350G was the most common variant (50%) followed by the European prototype (40.3%) and the North‐American (N = 3; 4.8%). The European prototype was significantly more frequent in patients infected with HPV16 alone (P < 0.05, C.I. 1.2–13.6), while the European T350G variants were seen in women with co‐infections. The North‐American lineage was found in women infected with HPV16 and 33. Three novel variants were identified of which two were non‐synonymous. Phylogenetic analysis revealed that the variant F69L + L83V is not related to any of these lineages, while the variant M137L + L83V is closely related to the North American variant. This study found a difference in the prevalence of E6 variants compared to earlier Indian studies and their association with type of infection. J. Med. Virol. 86:968–974, 2014. © 2014 Wiley Periodicals, Inc. |
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Bibliography: | National Institute for Research in Reproductive Health to conduct the research project istex:9451F7E899740FB02BF4BEA8A1CFFB1517FC7AE4 ark:/67375/WNG-3MK90NQX-V ArticleID:JMV23924 TATA Memorial Hospital, Mumbai for their Clinical support ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 0146-6615 1096-9071 |
DOI: | 10.1002/jmv.23924 |