The oncogenic role of androgen receptors in promoting the growth of oral squamous cell carcinoma cells
Objectives The aims of this study were to examine the expression of androgen receptors (AR) in oral squamous cell carcinoma (OSCC) cells and tumors and to determine the role of AR in regulating OSCC cell growth. Materials and Methods Four OSCC cell lines were used for analyzing AR expression and tra...
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Published in | Oral diseases Vol. 21; no. 3; pp. 320 - 327 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Denmark
Blackwell Publishing Ltd
01.04.2015
Wiley Subscription Services, Inc |
Subjects | |
Online Access | Get full text |
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Summary: | Objectives
The aims of this study were to examine the expression of androgen receptors (AR) in oral squamous cell carcinoma (OSCC) cells and tumors and to determine the role of AR in regulating OSCC cell growth.
Materials and Methods
Four OSCC cell lines were used for analyzing AR expression and transcriptional activity. The effects of AR knockdown on the growth and tumorigenicity of OSCC cells were examined. A series of 11 benign, 22 premalignant, and 21 malignant lesions of the oral cavity were used for analyzing AR expression.
Results
OSCC cells expressed AR proteins with differential activities. Stimulation of AR by dihydrotestosterone in OSCC cells caused an increase in cyclin D1 expression and promoted cell growth, whereas treatment with bicalutamide led to decreased cyclin D1 expression and inhibited cell growth. Knockdown of AR expression in OSCC cells resulted in decreased proliferation, increased apoptosis, and inhibited tumorigenicity. Results from immunohistochemical studies showed that AR immunoreactivity was found in 27% (3/11) of benign lesions, while 68% (15/22) of premalignant and 67% (14/21) of malignant lesions showed positive AR staining.
Conclusion
Our data suggest that OSCC cells express functional AR proteins which are critical for promoting cell growth and causing malignant disease. |
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Bibliography: | istex:4A96DF8EC2E915D8CD89E5886A51BBBD6F3B4709 Department of Health, Executive Yuan, Taiwan - No. DOH 102-TD-C-111-008 ArticleID:ODI12272 Taiwan National Science Council - No. NSC 98-2314-B-259-001; No. NSC 99-2320-B-259-001-MY3 Table S1 Consistency of visual classification between pathologists. Table S2 Kappa indices for interobserver reliability among pairs of pathologists. ark:/67375/WNG-Z50GCMWC-H ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1354-523X 1601-0825 |
DOI: | 10.1111/odi.12272 |