A pilot study of estrogen's effects on bronchial myocyte adhesion molecule expression

We examined the effects of estrogen on tumor necrosis factor alpha (TNF-alpha)-induced expression of intracellular adhesion molecule (ICAM-1) and vascular adhesion molecule (VCAM-1) in cultured human bronchial smooth muscle cells (BSMC). Experiments were performed in triplicate in T-75 tissue cultur...

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Bibliographic Details
Published inPharmacotherapy Vol. 19; no. 12; p. 1426
Main Authors Dickens, G R, Matheny, C J, Morris, P E, Clifton, G D, Ensom, M H
Format Journal Article
LanguageEnglish
Published United States 01.12.1999
Subjects
Bronchi - drug effects
Bronchi - metabolism
Cells, Cultured
Colforsin - pharmacology
Cyclic AMP - biosynthesis
Estradiol - pharmacology
Flow Cytometry
Humans
Intercellular Adhesion Molecule-1 - metabolism
Muscle, Smooth - drug effects
Muscle, Smooth - metabolism
Pilot Projects
Radioimmunoassay
Time Factors
Tumor Necrosis Factor-alpha - pharmacology
Vascular Cell Adhesion Molecule-1 - metabolism
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Summary:We examined the effects of estrogen on tumor necrosis factor alpha (TNF-alpha)-induced expression of intracellular adhesion molecule (ICAM-1) and vascular adhesion molecule (VCAM-1) in cultured human bronchial smooth muscle cells (BSMC). Experiments were performed in triplicate in T-75 tissue culture flasks containing normal human BSMC. Four experiments were carried out: untreated BSMC cells (control); TNF-alpha 1000 U/ml stimulation of BSMC; forskolin 5 microM before TNF-alpha stimulation of BSMC; and estradiol 30 microM before TNF-alpha stimulation of BSMC. Cyclic adenosine monophosphate was measured by a commercially available radioimmunoassay kit. Cell expression of ICAM-1 and VCAM-1 was quantified by flow cytometry Incubation of cells with TNF-alpha 1000 U/ml for 24 hours elicited a 27-fold increase in basal expression of ICAM-1 and a 2-fold increase in VCAM-1 (p>0.05). Incubation of BSMC with forskolin 5 microM, for 1 hour before TNF-alpha, decreased TNF-alpha-induced expression of ICAM-1 by 62% and VCAM-1 slightly by 17%. The BSMC incubated with estradiol 30 microM, 1 hour before TNF-alpha, decreased TNF-alpha-induced expression of ICAM-1 by 21%; VCAM-1 remained unchanged (p>0.05). We found a trend toward inhibition of TNF-alpha-stimulated ICAM-1 expression in cultured BSMC with pretreatment with estradiol. However, due to large variability within the cell culture model, statistical significance was not reached.
ISSN:0277-0008
DOI:10.1592/phco.19.18.1426.30902