Interaction of a copper(II)–Schiff base complexes with calf thymus DNA and their antimicrobial activity

• Published in: International journal of biological macromolecules Vol. 48; no. 2; pp. 319 - 325
• Main Authors: Sabolová, D., Kožurková, M., Plichta, T., Ondrušová, Z., Hudecová, D., Šimkovič, M., Paulíková, H., Valent, A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2011
• Subjects: Animals; Anti-Infective Agents - pharmacology; anti-infective properties; Antimicrobial activity; ascorbic acid; Ascorbic Acid - pharmacology; calves; Candida albicans; Candida albicans - drug effects; Candida albicans - growth & development; Cattle; Circular Dichroism; copper; Copper - metabolism; Copper - physiology; Cu(II) complex; DNA; DNA - metabolism; DNA binding; Electrons; fluorescence; free radicals; Intracellular Space - metabolism; Microbial Sensitivity Tests; Microscopy, Fluorescence; Oxidative stress; Plasmids; Reactive Oxygen Species; schiff bases; Schiff Bases - metabolism; Schiff Bases - pharmacology; Spectrometry, Fluorescence; spectroscopy; synergism; thymus gland; Titrimetry; Oxidative stress; Cu(II) complex; Antimicrobial activity; DNA binding
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2010.12.001

The interaction of a copper complexes containing Schiff bases with calf thymus (CT) DNA was investigated by spectroscopic methods. UV–vis, fluorescence and CD spectroscopies were conducted to assess their binding ability with CT DNA. The binding constants K have been estimated from 0.8 to 9.1 × 10 4 M −1. The percentage of hypochromism is found to be over 70% (from spectral titrations). The results showed that the copper(II) complexes could bind to DNA with an intercalative mode. Synergic action of Cu(II) complexes with ascorbic acid against Candida albicans induced the generation of free radicals and increased (more than 60 times) antimicrobial effect of these complexes.