Platelets as a source of fibroblast growth-promoting activity

Evidence is presented that platelets are an enriched source of growth-promoting activity for 3T3 cells, an established line of mouse fibroblasts. Rat or human serum stimulates 3T3 cell growth more than autologous plasma; this increased activity is at least partly due to the release of a growth-promo...

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Bibliographic Details
Published inExperimental cell research Vol. 87; no. 2; pp. 297 - 301
Main Authors Kohler, Nancy, Lipton, A.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.08.1974
Subjects
Animals
Blood
Blood Platelets
Cell Division
Cell Transformation, Neoplastic
Cells, Cultured
Fibroblasts
Hot Temperature
Hydrogen-Ion Concentration
Mice
Molecular Weight
Pepsin A - pharmacology
Rats
Simian virus 40
Stimulation, Chemical
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Summary:Evidence is presented that platelets are an enriched source of growth-promoting activity for 3T3 cells, an established line of mouse fibroblasts. Rat or human serum stimulates 3T3 cell growth more than autologous plasma; this increased activity is at least partly due to the release of a growth-promoting activity from platelets disrupted during the clotting process. Platelet extract stimulation of 3T3 cell growth is approx. 5 times greater than an equal amount of autologous serum. This platelet growth activity is (1) pH-stable; (2) pepsin-labile; (3) heat-stable at 56 °C; (4) non-dialysable, and (5) similar in size to the serum 3T3 growth-promoting molecule. 3T3 cells transformed by Simian virus 40 show an equivalent stimulation by the platelet solution and serum.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0014-4827
1090-2422
DOI:10.1016/0014-4827(74)90484-4