Micro- and nanofluidics for DNA analysis

Miniaturization to the micrometer and nanometer scale opens up the possibility to probe biology on a length scale where fundamental biological processes take place, such as the epigenetic and genetic control of single cells. To study single cells the necessary devices need to be integrated on a sing...

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Bibliographic Details
Published inAnalytical and bioanalytical chemistry Vol. 378; no. 7; pp. 1678 - 1692
Main Authors Tegenfeldt, Jonas O, Prinz, Christelle, Cao, Han, Huang, Richard L, Austin, Robert H, Chou, Stephen Y, Cox, Edward C, Sturm, James C
Format Journal Article
LanguageEnglish
Published Germany Springer-Verlag 01.04.2004
Subjects
Analytical Chemistry
Analytisk kemi
Cell Separation
Chemical Sciences
dielectrophoresis
DNA
DNA - analysis
DNA - genetics
epigenetics
gels
Kemi
Microfluidics
Nanotechnology
Natural Sciences
Naturvetenskap
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Summary:Miniaturization to the micrometer and nanometer scale opens up the possibility to probe biology on a length scale where fundamental biological processes take place, such as the epigenetic and genetic control of single cells. To study single cells the necessary devices need to be integrated on a single chip; and, to access the relevant length scales, the devices need to be designed with feature sizes of a few nanometers up to several micrometers. We will give a few examples from the literature and from our own research in the field of miniaturized chip-based devices for DNA analysis, including dielectrophoresis for purification of DNA, artificial gel structures for rapid DNA separation, and nanofluidic channels for direct visualization of single DNA molecules.
Bibliography:http://dx.doi.org/10.1007/s00216-004-2526-0
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ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-004-2526-0