Recognition sequences of repressor and polymerase in the operators of bacteriophage lambda

Nucleotide sequences in two wild-type and six mutant operators in the DNA of phage λ are compared. Strikingly similar 17 base pair units are found which we identify as the repressor binding sites. Each operator contains multiple repressor binding sites separated by A-T rich spacers. Elements of 2 fo...

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Bibliographic Details
Published inCell Vol. 5; no. 2; pp. 109 - 113
Main Authors Maniatis, Tom, Ptashne, Mark, Backman, Keith, Kleid, Dennis, Flashman, Stuart, Jeffrey, Andrea, Maurer, Russell
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.06.1975
Subjects
Base Sequence
Binding Sites
Chromosome Mapping
Coliphages - analysis
DNA, Viral - analysis
DNA-Directed RNA Polymerases
Escherichia coli - enzymology
Genes, Regulator
Genetic Code
Mutation
Operon
Transcription, Genetic
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Summary:Nucleotide sequences in two wild-type and six mutant operators in the DNA of phage λ are compared. Strikingly similar 17 base pair units are found which we identify as the repressor binding sites. Each operator contains multiple repressor binding sites separated by A-T rich spacers. Elements of 2 fold rotational symmetry are present in each of the sites. Superimposed on each operator is an E. coli RNA polymerase recognition site (promoter). Similarities in the sequences of the two λ promoters, a lac promoter, and an E. coli RNA polymerase recognition site in SV40 DNA are noted.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0092-8674
1097-4172
DOI:10.1016/0092-8674(75)90018-5