An Efficient Deacidification Process for Safflower Seed Oil with High Nutritional Property through Optimized Ultrasonic-Assisted Technology

• Published in: Molecules (Basel, Switzerland) Vol. 27; no. 7; p. 2305
• Main Authors: Xin, Leyu, Guo, Limin, Edirs, Salamet, Zhang, Zepeng, Cai, Chenyang, Yang, Yongxing, Lian, Yali, Yang, Haiyan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.04.2022; MDPI
• Subjects: Alkalies; Anisidine; antioxidant capacity; Antioxidants; Bivariate analysis; Carthamus tinctorius - chemistry; Centroids; Correlation analysis; Deacidification; Edible oils; Ethanol; Ethanol - chemistry; Fatty acids; lipid concomitants; Lipids; Oils & fats; Oilseeds; Optimization; Oxidation; Peroxides; Physicochemical properties; Phytosterols; Plant Oils - chemistry; Polyphenols; random centroid optimization; Refining; Safflower Oil; safflower seed oil; Scavenging; Seeds; Technology; Tocopherols; ultrasonic-assisted ethanol extraction deacidification; Ultrasonics; Vegetable oils; Wastewater; safflower seed oil; lipid concomitants; ultrasonic-assisted ethanol extraction deacidification; antioxidant capacity; random centroid optimization
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27072305

Safflower seed oil (SSO) is considered to be an excellent edible oil since it contains abundant essential unsaturated fatty acids and lipid concomitants. However, the traditional alkali-refined deacidification process of SSO results in a serious loss of bioactive components of the oil and also yields massive amounts of wastewater. In this study, SSO was first extracted by ultrasonic-assisted ethanol extraction (UAEE), and the extraction process was optimized using random centroid optimization. By exploring the effects of ethanol concentration, solid−liquid ratio, ultrasonic time, and the number of deacidification times, the optimum conditions for the deacidification of safflower seed oil were obtained as follows: ethanol concentration 100%, solid−liquid ratio 1:4, ultrasonic time 29 min, and number of deacidification cycles (×2). The deacidification rate was 97.13% ± 0.70%, better than alkali-refining (72.16% ± 0.13%). The values of acid, peroxide, anisidine and total oxidation of UAEE-deacidified SSO were significantly lower than those of alkali-deacidified SSO (p < 0.05). The contents of the main lipid concomitants such as tocopherols, polyphenols, and phytosterols in UAEE-decidified SSO were significantly higher than those of the latter (p < 0.05). For instance, the DPPH radical scavenging capacity of UAEE-processed SSO was significantly higher than that of alkali refining (p < 0.05). The Pearson bivariate correlation analysis before and after the deacidification process demonstrated that the three main lipid concomitants in SSO were negatively correlated with the index of peroxide, anisidine, and total oxidation values. The purpose of this study was to provide an alternative method for the deacidification of SSO that can effectively remove free fatty acids while maintaining the nutritional characteristics, physicochemical properties, and antioxidant capacity of SSO.