Effects of the fungicide tebuconazole on microbial capacities for litter breakdown in streams

• Published in: Aquatic toxicology Vol. 122-123; pp. 197 - 205
• Main Authors: Artigas, Joan, Majerholc, Joy, Foulquier, Arnaud, Margoum, Christelle, Volat, Bernadette, Neyra, Marc, Pesce, Stéphane
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.10.2012; Elsevier
• Subjects: Alnus - metabolism; Alnus - microbiology; Alnus glutinosa; Artificial streams; Bacteria; Bacteria - drug effects; Bacteria - enzymology; basins; Biodiversity; Biomass; cellulose; Cellulose - metabolism; chronic exposure; community structure; Environmental Sciences; enzymes; Enzymes - metabolism; Extracellular enzyme kinetics; Fresh Water - chemistry; Fungi; Fungi - drug effects; Fungi - enzymology; Fungicides, Industrial - toxicity; leaves; microbial biomass; microbial communities; Plant Leaves - metabolism; Plant Leaves - microbiology; plant litter; Polysaccharides - metabolism; Populus - metabolism; Populus - microbiology; Populus nigra; Rivers - microbiology; stream channels; tebuconazole; Time Factors; Triazoles - toxicity; Water Pollutants, Chemical - toxicity; Fungi; Alnus glutinosa; Extracellular enzyme kinetics; Bacteria; Artificial streams; Populus nigra; POPULUS NIGRA; ARTIFICIAL STREAMS; BACTERIA; FUNGI; ALNUS GLUTINOSA; EXTRACELLULAR ENZYME KINETICS
• ISSN: 0166-445X; 1879-1514
• DOI: 10.1016/j.aquatox.2012.06.011

► The impact of tebuconazole was analyzed on the breakdown of Alnus glutinosa and Populus nigra litter by aquatic microorganisms. ► Litter breakdown rates of Alnus and Populus species were reduced in presence of the fungicide. ► The fungicide inhibited the development of fungal and bacterial biomass and produced changes in communities’ composition. ► Fungal and bacterial responses to TBZ were influenced by the quality of litter substrates. ► The chronic exposure to TBZ can affect aquatic microbial communities and their capacity to recycle organic matter. Streams draining agricultural basins are subjected to the input of fungicides which can affect aquatic microbial communities. We analyzed the effect of the fungicide tebuconazole (TBZ) on Alnus glutinosa and Populus nigra litter breakdown by aquatic microorganisms. For six weeks, fungal and bacterial responses were analyzed in indoor stream channels subjected to TBZ-contaminated (33.1±12.4μgL−1) and uncontaminated conditions. Litter breakdown rates decreased in presence of TBZ. The decrease was explained by reductions in microbial biomass development and shifts in community structure. At the same time, TBZ modified the kinetics of β-glucosidase, β-xylosidase and cellobiohydrolase enzymes resulting in lower affinities for cellulose and hemicellulose decomposition in leaves. These alterations were modulated by the litter quality; the greatest structural impairment was observed in Populus whereas Alnus were more affected in terms of leaf breakdown rate. Our results suggest that chronic exposure to TBZ can affect aquatic microbial communities and their capacity to break down leaf litter in streams.