Crataegus laevigata Suppresses LPS-Induced Oxidative Stress during Inflammatory Response in Human Keratinocytes by Regulating the MAPKs/AP-1, NFκB, and NFAT Signaling Pathways

• Published in: Molecules (Basel, Switzerland) Vol. 26; no. 4; p. 869
• Main Authors: Nguyen, Quynh T N, Fang, Minzhe, Zhang, Mengyang, Do, Nhung Quynh, Kim, Minseon, Zheng, Sheng Dao, Hwang, Eunson, Yi, Tae Hoo
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 06.02.2021; MDPI
• Subjects: Acids; Activator protein 1; Cell Survival - drug effects; Chemokines; Chemokines - genetics; Crataegus - chemistry; Crataegus laevigata; Cytokines; Cytotoxicity; Ethanol; Gene Expression Regulation - drug effects; Humans; Inflammation; Inflammation - metabolism; Inflammation - pathology; Inflammatory response; Interleukins; Keratinocytes; Keratinocytes - drug effects; Keratinocytes - metabolism; Keratinocytes - pathology; Kinases; Ligands; Lipopolysaccharides; Lipopolysaccharides - pharmacology; LPS; MAP kinase; MAP Kinase Signaling System - drug effects; MAPK/AP-1; mRNA; NF-AT protein; NF-kappa B - metabolism; NF-κB protein; NFATC Transcription Factors - metabolism; Oxidative stress; Oxidative Stress - drug effects; Phosphorylation; Plant Extracts - pharmacology; Proteins; RNA, Messenger - genetics; Signal transduction; Skin; Transcription Factor AP-1 - metabolism; Transcription factors; Europe; NF-κB; inflammation; MAPK/AP-1; keratinocytes; Crataegus laevigata; LPS; oxidative stress; NFAT
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules26040869

belongs to the family Rosaceae, which has been widely investigated for pharmacological effects on the circulatory and digestive systems. However, there is limited understanding about its anti-oxidative stress and anti-inflammatory effects on skin. In this study, 70% ethanol berry extract (CLE) was investigated on lipopolysaccharide (LPS)-stimulated keratinocytes. The LPS-induced overproduction of reactive oxygen species (ROS) was suppressed by the treatment with CLE. In response to ROS induction, the overexpression of inflammatory regulating signaling molecules including mitogen-activated protein kinases (MAPK)/activator protein-1 (AP-1), nuclear factor kappa-light-chain-enhancer of activated B cell (NF-κB), and nuclear factor of activated T-cells (NFAT) were reduced in CLE-treated human keratinocytes. Consequently, CLE significantly suppressed the mRNA levels of pro-inflammatory chemokines and interleukins in LPS-stimulated cells. Our results indicated that CLE has protective effects against LPS-induced injury in an in vitro model and is a potential alternative agent for inflammatory treatment.