Association of promoter polymorphism of the CD14 C (-159) T endotoxin receptor gene with chronic hepatitis B

• Published in: World journal of gastroenterology : WJG Vol. 12; no. 35; pp. 5717 - 5720
• Main Authors: Mohammad Alizadeh, Amir Houshang, Ranjbar, Mitra, Hajilooi, Mehrdad, Fallahian, Farahnaz
• Format: Journal Article
• Language: English
• Published: United States Research Center for Gastroenterology and Liver Disease, Shaheed Beheshti University of Medical Sciences, Tehran, Iran%Hamedan University of Medical Sciences Hamedan, Iran 21.09.2006; Baishideng Publishing Group Co., Limited
• Subjects: Adult; Alleles; Case-Control Studies; DNA - genetics; Female; Genetic Predisposition to Disease; Genotype; Hepatitis B, Chronic - etiology; Hepatitis B, Chronic - genetics; Heterozygote; Homozygote; Humans; Lipopolysaccharide Receptors - genetics; Male; Middle Aged; Polymorphism, Single Nucleotide - genetics; Promoter Regions, Genetic - genetics; Rapid Communication; Receptors, Immunologic - genetics; Single nucleotide polymorphism; Chronic hepatitis B; Endotoxin susceptibility; CD14 C (-159) T gene
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v12.i35.5717

To investigate whether single-nucleotide polymorphisms in the promoter regions of endotoxin-responsive genes CD14 C (-159) T is associated with chronic hepatitis B. We obtained genomic DNA from 80 patients with established diagnosis of chronic hepatitis B and 126 healthy subjects served as a control population. The CD 14 C (-159) T polymorphism was investigated using an allele specific PCR method. Twenty seven percent of chronic hepatitis B patients and 75% of controls were heterozygous for CT genotype. The difference between the chronic hepatitis B and control groups was statistically significant [P < 0.0001; Odds ratio (OR) = 2.887; 95% CI: 1.609-5.178]. Twenty four point six percent of chronic hepatitis B and patients 12.3% of the control group were heterozygous for TT genotype. The difference between groups was not statistically significant (P = 0.256; OR = 0.658; 95% CI: 0.319-1.358). Forty eight point four percent of chronic hepatitis B patients and 12.7% of control were homozygote for CC genotype (P < 0.004; OR = 0.416; 95% CI: 0.229-0.755). The frequency of allele C was 61.9% and allele T was 38.1% in hepatitis B patients group. The frequency of allele C was 55.2% and allele T was 44.8% for the control group (P = 0.179; OR = 1.319; 95% CI: 0.881-1.977). The TT heterozygous genotype was not a risk factor for chronic hepatitis B. CC homozygote genotype is protective for hepatitis B. Lack of heterozygosis of genotype CT is a risk factor for chronic hepatitis B. Alleles C or T were not risk factors for chronic hepatitis B. These findings show the role of a single-nucleotide polymorphism at CD14/-159 on the development of chronic hepatitis B. Endotoxin susceptibility may play a role in the pathogenesis of chronic hepatitis B.