Kitasatospora viridis sp. nov., a novel actinomycete from soil

• Published in: International journal of systematic and evolutionary microbiology Vol. 55; no. 2; pp. 707 - 711
• Main Authors: Liu, Zhiheng, Rodriguez, Carlos, Wang, Liming, Cui, Quingfeng, Huang, Ying, Quintana, Erika T, Goodfellow, Michael
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.03.2005; Society for General Microbiology
• Subjects: Bacteriology; Biological and medical sciences; Camellia - microbiology; China; DNA, Bacterial - analysis; DNA, Ribosomal - analysis; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Genotype; Kitasatospora arboriphila; Kitasatospora viridis; Microbiology; Molecular Sequence Data; Phenotype; Phylogeny; Plant Roots - microbiology; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Streptomycetaceae - classification; Streptomycetaceae - genetics; Streptomycetaceae - isolation & purification; Streptomycetaceae - physiology; Systematics; China; Rhizosphere; Soils; Plant; Taxonomy; Bacteria; Actinomycetes; New species
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.63329-0

1 State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China 2 School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK Correspondence Michael Goodfellow m.goodfellow{at}ncl.ac.uk The taxonomic position of a rhizosphere isolate, strain 52108a T , was determined using a polyphasic approach. The strain was found to have chemical and morphological properties consistent with its assignment to the genus Kitasatospora . An almost complete 16S rRNA gene sequence determined for the strain was aligned with corresponding sequences of representatives of the genus Kitasatospora and related taxa using three tree-making algorithms. The organism formed a distinct phyletic line within the Kitasatospora clade and was most closely related to Kitasatospora arboriphila (98·9 %), Kitasatospora kifunensis (99·0 %), Kitasatospora paracochleata (98·4 %) and Kitasatospora terrestris (98·2 %), but was readily distinguished from representatives of these species using a combination of phenotypic properties. The combined genotypic and phenotypic data show that the strain should be classified in the genus Kitasatospora as a novel species. The name proposed is Kitasatospora viridis sp. nov., with the type strain 52108a T (=AS 4.1878 T =DSM 44826 T ). Published online ahead of print on 3 December 2004 as DOI 10.1099/ijs.0.63329-0. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain 52108a T is AY613990 .