Characterization of the mouse JAB1 cDNA and protein

• Published in: Gene Vol. 242; no. 1; pp. 41 - 50
• Main Authors: Bounpheng, Mangkey A., Melnikova, Irena N., Dodds, Sherry G., Chen, Heping, Copeland, Neal G., Gilbert, Debra J., Jenkins, Nancy A., Christy, Barbara A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 25.01.2000
• Subjects: Animals; c-Jun gene; c-Jun protein; Cell Line; Cell Nucleus - chemistry; Chromosomal location; chromosome 1; Chromosome Mapping; COP9 Signalosome Complex; Cytoplasm - chemistry; DNA, Complementary - chemistry; DNA, Complementary - genetics; DNA, Complementary - isolation & purification; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Embryo, Mammalian - metabolism; Female; Fluorescent Antibody Technique; Gene Expression Regulation; Gene Expression Regulation, Developmental; Humans; Hydrolysis; Intracellular Signaling Peptides and Proteins; JAB1 protein; JunD gene; JunD protein; Male; Mice; Mice, Inbred C57BL; Molecular Sequence Data; mRNA; Muridae; Oligopeptides; Peptide Hydrolases - metabolism; Peptides - genetics; Proteasome; Proteasome Endopeptidase Complex; Protein expression; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; RNA - genetics; RNA - metabolism; Sequence Analysis, DNA; Subcellular location; Tissue Distribution; Transcription Factors - genetics; Transcription Factors - metabolism; Ubiquitin; Ubiquitins - metabolism; Ubiquitin; AJH, Arabidopsis JAB1 homologue; MAP kinase, mitogen activated protein kinase; FBS, fetal bovine serum; mRNA; DMEM, Dulbecco's minimal essential medium; SDS, sodium dodecyl sulfate; DAPI, 4,6–Diamidino–2–phenylindole; HLH, helix–loop–helix; SAPK/JNK, stree activated protein kinase/Jun N-terminal kinase; RFLP, restriction fragment length polymorphism; Subcellular location; Chromosomal location; Protein expression; PCR, polymerase chain reaction; PAGE, polyacrylimide gel electrophoresis; mJAB1, murine Jun activation domain binding protein 1; Proteasome
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/S0378-1119(99)00525-9

JAB1 was originally described as a transcriptional coactivator of c-Jun and Jun D. Recent data suggests that JAB1 is a component of a large protein complex, the JAB1 signalosome in mammals and the COP9 complex in plants. The JAB1 signalosome is implicated in the phosphorylation of selected transcription factors, while the COP9 complex is involved in repression of photomorphogenesis in Arabidopsis. In this study, we describe the partial characterization of mouse JAB1 (mJAB1). The murine JAB1 protein is encoded by a gene located on mouse chromosome 1. mJAB1 mRNA is abundantly expressed in a variety of adult tissues as well as in mouse embryos. The JAB1 protein was readily detectable in many cell types and localized to both the nucleus and cytoplasm. Endogenous JAB1 protein is relatively stable and its degradation is not perturbed by blocking 26S proteasome activity, suggesting that this protein is not degraded by the ubiquitin-mediated proteolytic pathway.