Surface Display of Heme- and Diflavin-Containing Cytochrome P450 BM3 in Escherichia coli: A Whole-Cell Biocatalyst for Oxidation

• Published in: Journal of microbiology and biotechnology Vol. 20; no. 4; pp. 712 - 717
• Main Authors: YIM, Sung-Kun, KIM, Dong-Hyun, JUNG, Heung-Chae, PAN, Jae-Gu, KANG, Hyung-Sik, AHN, Taeho, YUN, Chul-Ho
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.04.2010; 한국미생물·생명공학회
• Subjects: Bacterial Outer Membrane Proteins - genetics; Bacterial Outer Membrane Proteins - metabolism; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biological and medical sciences; Biotechnology; Catalysis; Cytochrome P-450 Enzyme System - genetics; Cytochrome P-450 Enzyme System - metabolism; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Escherichia coli - enzymology; Escherichia coli - genetics; Escherichia coli - metabolism; Flavins - genetics; Flavins - metabolism; Flow Cytometry; Fundamental and applied biological sciences. Psychology; Heme - genetics; Heme - metabolism; NADPH-Ferrihemoprotein Reductase - genetics; NADPH-Ferrihemoprotein Reductase - metabolism; Oxidation-Reduction; Plasmids - genetics; Plasmids - metabolism; Polymerase Chain Reaction; Spectrophotometry, Ultraviolet; 생물학; P450 BM3; Cell oxidation; Cytochrome P450; Escherichia coli; whole-cell biocatalyst; Heme; oxidation; surface display; Bacteria; Enterobacteriaceae; Biocatalyst
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.0910.10043

Cytochrome P450 enzymes (P450s) are involved in the synthesis of a wide variety of valuable products and in the degradation of numerous toxic compounds. The P450 BM3 (CYP102A1) from Bacillus megaterium was the first P450 discovered to be fused to its redox partner, a mammalian-like diflavin reductase. Here, we report the development of a whole cell biocatalyst using ice-nucleation protein (Inp) from Pseudomonas syringae to display a heme- and diflavin-containing oxidoreductase, P450 BM3 (a single, 119-kDa polypeptide with domains of both an oxygenase and a reductase) on the surface of Escherichia coli. Surface localization and functionality of the fusion protein containing P450 BM3 were verified by flow cytometry and measurement of enzymatic activities. The results of this study comprise the first report of microbial cell-surface display of heme- and diflavin-containing enzyme. This system should allow us to select and develop oxidoreductases containing heme and/or flavins, into practically useful whole-cell biocatalysts for extensive biotechnological applications including selective synthesis of new chemicals and pharmaceuticals, bioconversion, bioremediation, live vaccine development, and bio-chip development.