Fluorometric determination of selenium in nanogram amounts in biological materials using 2,3-diaminonaphthalene

Nanogram amounts of selenium in biological material were determined fluorometrically using 2,3-diaminonaphthalene after digestion with nitric-perchloric acids. Linear relationship between fluorescence and selenium concentration held within 100 ng of selenium, and the experimental scattering was less...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 126; no. 2; pp. 242 - 245
Main Authors Hasunuma, Ryoichi, Ogawa, Tadao, Kawanishi, Yasuhiro
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.11.1982
Subjects
2-Naphthylamine - analogs & derivatives
Animals
Fishes
Fluorometry - methods
Muscles - analysis
Perchlorates
Selenium - analysis
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Summary:Nanogram amounts of selenium in biological material were determined fluorometrically using 2,3-diaminonaphthalene after digestion with nitric-perchloric acids. Linear relationship between fluorescence and selenium concentration held within 100 ng of selenium, and the experimental scattering was less than 4% for a range from 5 to 25 ng of selenium. A recovery of 99 ± 5% was achieved for 2.5 ng of selenium preadded to tuna muscle extract. The method is characterized by simple procedures and is suited for small quantities of samples.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(82)90510-3