High-level conjugation of chelating agents onto immunoglobulins: use of an intermediary poly( l-lysine)-diethylenetriaminepentaacetic acid carrier
Diethylenetriaminepentaacetic acid (DTPA), a strong chelating agent, was covalently linked to murine monoclonal anti-HLA IgG 1 antibody (H-1) with the use of poly( l-lysine) ( M r 14 000) as a multivalent, intermediary carrier, via thiol-disulfide exchange reaction. The conjugates contained up to 42...
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Published in | Biochimica et biophysica acta Vol. 883; no. 3; pp. 460 - 467 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
01.10.1986
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Subjects | |
Online Access | Get full text |
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Summary: | Diethylenetriaminepentaacetic acid (DTPA), a strong chelating agent, was covalently linked to murine monoclonal anti-HLA IgG
1 antibody (H-1) with the use of poly(
l-lysine) (
M
r 14 000) as a multivalent, intermediary carrier, via thiol-disulfide exchange reaction. The conjugates contained up to 42.5 mol DTPA per mol antibody, and retained over 90% of their antibody activity in vitro. The conjugates incorporated gadolinium (Gd) through an exchange reaction with Gd-EDTA, used to prevent colloid formation and nonspecific binding of the free metal. The IgG-poly(
l-lysine)-DTPA-Gd had a greater effect per mol on proton relaxation rates than DTPA-Gd itself. Use of poly(
l-lysine) as an intermediary carrier for attachment of chelating agents to IgG thus offers great potential for achieving high-specific-activity conjugates, particularly for use as biologically specific contrast agents in nuclear magnetic resonance imaging. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0304-4165 0006-3002 1872-8006 |
DOI: | 10.1016/0304-4165(86)90285-0 |