Establishing a Symbiotic Interface between Cultured Ectomycorrhizal Fungi and Plants to Follow Fungal Phosphate Metabolism
In ectomycorrhizal plants, the fungal cells colonize the roots of their host plant to create new organs called ectomycorrhizae. In these new organs, the fungal cells colonize the walls of the cortical cells, bathing in the same apoplasm as the plant cells in a space named the 'Hartig net',...
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Published in | Bio-protocol Vol. 7; no. 20; p. e2577 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Bio-protocol LCC
20.10.2017
Bio-Protocol Bio-protocol LLC |
Subjects | |
Online Access | Get full text |
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Summary: | In ectomycorrhizal plants, the fungal cells colonize the roots of their host plant to create new organs called ectomycorrhizae. In these new organs, the fungal cells colonize the walls of the cortical cells, bathing in the same apoplasm as the plant cells in a space named the 'Hartig net', where exchanges between the two partners take place. Finally, the efficiency of ectomycorrhizal fungi to improve the phosphorus nutrition of their host plants will depend on the regulation of phosphate transfer from the fungal cells to plant cells in the Hartig net through as yet unknown mechanisms. In order to investigate these mechanisms, we developed an
experimental device mimicking the common apoplasm of the ectomycorrhizae (the Hartig net) to study the phosphorus metabolism in the ectomycorrhizal fungus
when the fungal cells are associated or not with the plant cells of the host plant
. This device can be used to monitor
Phosphate efflux from the fungus previously incubated with
P-orthophosphate. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Present address: Colegio de Postgraduados, Campus San Luis Potosí, Agustín de Iturbide Nº 73, Salinas de Hidalgo, Salinas, S.L.P, México, Mexico |
ISSN: | 2331-8325 2331-8325 |
DOI: | 10.21769/BioProtoc.2577 |