The Overexpression of Zea mays Strigolactone Receptor Gene D14 Enhances Drought Resistance in Arabidopsis thaliana L

• Published in: International journal of molecular sciences Vol. 25; no. 2; p. 1327
• Main Authors: Zhang, Chen, Wang, Fanhao, Jiao, Peng, Liu, Jiaqi, Zhang, Honglin, Liu, Siyan, Guan, Shuyan, Ma, Yiyong
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 22.01.2024
• Subjects: Abscisic acid; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis thaliana; Biosynthesis; D14; Drought; Drought Resistance; Droughts; Gene Expression Regulation, Plant; Genes; Heterocyclic Compounds, 3-Ring; Hormones; Investigations; Lactones; Physiology; Plant Proteins - genetics; Plant Proteins - metabolism; Plants, Genetically Modified - genetics; Plants, Genetically Modified - metabolism; Proteins; Salinity; Signal transduction; Sorghum; strigolactone; Zea mays; Zea mays - genetics; Zea mays - metabolism; Arabidopsis thaliana; Zea mays; strigolactone; D14
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25021327

Strigolactones (SLs) represent a recently identified class of plant hormones that are crucial for plant tillering and mycorrhizal symbiosis. The gene, an essential receptor within the SLs signaling pathway, has been well-examined in crops, like rice ( L.) and ( L.), yet the research on its influence in maize ( L.) remains scarce. This study successfully clones and establishes gene overexpression lines (OE lines). When compared with the wild type (WT), the OE lines exhibited significantly longer primary roots during germination. By seven weeks of age, these lines showed reductions in plant height and tillering, alongside slight decreases in rosette and leaf sizes, coupled with early aging symptoms. Fluorescence-based quantitative assays indicated notable hormonal fluctuations in OE lines versus the WT, implying that overexpression disrupts plant hormonal homeostasis. The OE lines, exposed to cold, drought, and sodium chloride stressors during germination, displayed an especially pronounced resistance to drought. The drought resistance of OE lines, as evident from dehydration-rehydration assays, outmatched that of the WT lines. Additionally, under drought conditions, the OE lines accumulated less reactive oxygen species (ROS) as revealed by the assessment of the related physiological and biochemical parameters. Upon confronting the pathogens pv. DC3000 ( DC3000), post-infection, fluorescence quantitative investigations showed a significant boost in the salicylic acid (SA)-related gene expression in OE lines compared to their WT counterparts. Overall, our findings designate the SL receptor as a key upregulator of drought tolerance and a regulator in the biotic stress response, thereby advancing our understanding of the maize SL signaling pathway by elucidating the function of the pivotal gene.