Artificial intelligence-driven drug repositioning uncovers efavirenz as a modulator of α-synuclein propagation: Implications in Parkinson’s disease

• Published in: Biomedicine & pharmacotherapy Vol. 174; p. 116442
• Main Authors: Kim, Jae-Bong, Kim, Soo-Jeong, So, Minyoung, Kim, Dong-Kyu, Noh, Hye Rin, Kim, Beom Jin, Choi, Yu Ree, Kim, Doyoon, Koo, Heejung, Kim, Taeyong, Woo, Hyun Goo, Park, Sang Myun
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.05.2024; Elsevier
• Subjects: Alkynes - pharmacology; alpha-Synuclein - metabolism; Animals; Artificial Intelligence; Benzoxazines - pharmacology; Caenorhabditis elegans - drug effects; Cyclopropanes - pharmacology; Cyclopropanes - therapeutic use; Disease Models, Animal; Drug repositioning; Drug Repositioning - methods; Efavirenz; Humans; Mice; Mice, Inbred C57BL; Mice, Transgenic; Nevirapine - pharmacology; Parkinson Disease - drug therapy; Parkinson Disease - metabolism; Parkinson’s disease; α-synuclein; Drug repositioning; Efavirenz; Parkinson’s disease; α-synuclein
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/j.biopha.2024.116442

Parkinson's disease (PD) is a complex neurodegenerative disorder with an unclear etiology. Despite significant research efforts, developing disease-modifying treatments for PD remains a major unmet medical need. Notably, drug repositioning is becoming an increasingly attractive direction in drug discovery, and computational approaches offer a relatively quick and resource-saving method for identifying testable hypotheses that promote drug repositioning. We used an artificial intelligence (AI)-based drug repositioning strategy to screen an extensive compound library and identify potential therapeutic agents for PD. Our AI-driven analysis revealed that efavirenz and nevirapine, approved for treating human immunodeficiency virus infection, had distinct profiles, suggesting their potential effects on PD pathophysiology. Among these, efavirenz attenuated α-synuclein (α-syn) propagation and associated neuroinflammation in the brain of preformed α-syn fibrils-injected A53T α-syn Tg mice and α-syn propagation and associated behavioral changes in the C. elegans BiFC model. Through in-depth molecular investigations, we found that efavirenz can modulate cholesterol metabolism and mitigate α-syn propagation, a key pathological feature implicated in PD progression by regulating CYP46A1. This study opens new avenues for further investigation into the mechanisms underlying PD pathology and the exploration of additional drug candidates using advanced computational methodologies. [Display omitted] •Our AI analysis shows that efavirenz and nevirapine, used in HIV treatment, may impact PD pathophysiology.•In vivo and in vitro, efavirenz attenuates α-syn propagation, micro/astrogliosis, and inhibits α-syn release and uptake.•RNA-seq analysis shows efavirenz modulates cholesterol metabolism, mitigating α-syn propagation in PD via CYP46A1 regulation.