Effects of 3-month high-intensity interval training vs. moderate endurance training and 4-month follow-up on fat metabolism, cardiorespiratory function and mitochondrial respiration in obese adults

• Published in: European journal of applied physiology Vol. 120; no. 8; pp. 1787 - 1803
• Main Authors: Vaccari, Filippo, Passaro, Angelina, D’Amuri, Andrea, Sanz, Juana Maria, Di Vece, Francesca, Capatti, Eleonora, Magnesa, Benedetta, Comelli, Marina, Mavelli, Irene, Grassi, Bruno, Fiori, Federica, Bravo, Giulia, Avancini, Alice, Parpinel, Maria, Lazzer, Stefano
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2020; Springer Nature B.V
• Subjects: Adult; Aerobics; Biomedical and Life Sciences; Biomedicine; Body composition; Body fat; Body mass index; Cardiorespiratory Fitness; Cell Respiration; Citrate synthase; Endurance Training - methods; Fat metabolism; Female; High-Intensity Interval Training - methods; Human Physiology; Humans; Interval training; Lipid Metabolism; Male; Middle Aged; Mitochondria; Mitochondria - metabolism; Obesity; Obesity - metabolism; Obesity - physiopathology; Obesity - therapy; Occupational Medicine/Industrial Medicine; Original Article; Oxidation; Oxygen Consumption; Respiration; Sports Medicine; Mitochondrial respiration; Obesity; HIIT; Aerobic function; Lipid oxidation
• ISSN: 1439-6319; 1439-6327; 1439-6327
• DOI: 10.1007/s00421-020-04409-2

Purpose The purpose of this study was to investigate, in obese adults, changes in body composition, physical capacities, fat oxidation and ex vivo mitochondrial respiration induced by a 3-month either moderate-intensity continuous training (MICT) or high-intensity interval training (HIIT); afterwards, the patients were followed for four months. Methods Thirty-two patients (mean age 39 years; mean body mass index [BMI] 36 kg∙m −2 ) participated in this study attending ~ 34 sessions of training. At baseline (PRE), at the end of the program (POST) and after follow-up, body composition, peak O 2 uptake (V′O 2 peak) and fat oxidation rate were measured. Vastus lateralis biopsies for the evaluation of mitochondrial respiration were performed only at PRE and POST. Results At POST, body mass (BM) and fat mass (FM) decreased (− 6 and − 14%, respectively, P  < 0.05) in MICT and HIIT; V′O 2 peak increased in both groups (+ 6 and + 16%, respectively, P  < 0.05). Maximal fat oxidation rate increased only after HIIT ( P  < 0.001). Maximal ADP-stimulated mitochondrial respiration normalized by citrate synthase increased ( P  < 0.05) by 67% and 36% in MICT and HIIT, respectively, without significant difference. After follow-up, BM and FM were still lower (− 4 and − 20%, respectively, P  < 0.050) compared with baseline in both groups. Only after HIIT, V′O 2 peak (+ 8%) and maximal fat oxidation rate were still higher ( P  < 0.05). Conclusions HIIT was more effective in improving and maintaining V’O 2 peak and fat oxidation. These results may be relevant for an appropriate prescription of training programs designed to optimize aerobic fitness in obese subjects.