One-step purification of mouse monoclonal antibodies from ascitic fluid by DEAE Affi-Gel blue chromatography

Monoclonal antibodies can be purified directly from ascitic fluids by chromatography on a DEAE Affi-gel blue column. Optimal conditions were determined for the recovery of immunoglobulins free of contaminating protease and nuclease activities.

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Bibliographic Details
Published inJournal of immunological methods Vol. 53; no. 3; p. 313
Main Authors Bruck, C, Portetelle, D, Glineur, C, Bollen, A
Format Journal Article
LanguageEnglish
Published Netherlands 30.09.1982
Subjects
Animals
Anthracenes
Antibodies, Monoclonal - isolation & purification
Ascitic Fluid - analysis
Chromatography, Gel
Chromatography, Ion Exchange
Hydrogen-Ion Concentration
Methods
Mice
Triazines
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Summary:Monoclonal antibodies can be purified directly from ascitic fluids by chromatography on a DEAE Affi-gel blue column. Optimal conditions were determined for the recovery of immunoglobulins free of contaminating protease and nuclease activities.
ISSN:0022-1759
DOI:10.1016/0022-1759(82)90178-8