Refined glufosinate selection in Agrobacterium -mediated transformation of soybean [ Glycine max (L.) Merrill]

Modern genetic analysis and manipulation of soybean ( Glycine max) depend heavily on an efficient and dependable transformation process, especially in public genotypes from which expressed sequence tag (EST), bacterial artificial chromosome and microarray data have been derived. Williams 82 is the s...

Full description

Saved in:
Bibliographic Details
Published inPlant cell reports Vol. 22; no. 7; pp. 478 - 482
Main Authors Zeng, P., Vadnais, D. A., Zhang, Z., Polacco, J. C.
Format Journal Article
LanguageEnglish
Published Germany Springer Nature B.V 01.02.2004
Subjects
Agrobacterium
Aminobutyrates
Aminobutyrates - pharmacology
Antioxidants
Antioxidants - pharmacology
Artificial chromosomes
bacterial artificial chromosomes
cysteine
drug effects
Embryonic growth stage
expressed sequence tags
Genes
genetics
Genomics
genotype
Genotypes
glufosinate
Glycine max
Glycine max - drug effects
Glycine max - genetics
Herbicides
Herbicides - pharmacology
microarray technology
pharmacology
Plants, Genetically Modified
Rhizobium
Rhizobium - genetics
Soybeans
Transformation, Genetic
Transformation, Genetic - drug effects
transgenic plants
Online AccessGet full text

Cover

More Information
Summary:Modern genetic analysis and manipulation of soybean ( Glycine max) depend heavily on an efficient and dependable transformation process, especially in public genotypes from which expressed sequence tag (EST), bacterial artificial chromosome and microarray data have been derived. Williams 82 is the subject of EST and functional genomics analyses. However, it has not previously been transformed successfully using either somatic embryogenesis-based or cotyledonary-node transformation methods, the two predominant soybean transformation systems. An advance has recently been made in using antioxidants to enhance Agrobacterium infection of soybean. Nonetheless, an undesirable effect of using these antioxidants is the compromised recovery of transgenic soybean when combined with the use of the herbicide glufosinate as a selective agent. Therefore, we optimized both Agrobacterium infection and glufosinate selection in the presence of L-cysteine for Williams 82. We have recovered transgenic lines of this genotype with an enhanced transformation efficiency using this herbicide selection system.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ObjectType-Article-2
ObjectType-Feature-1
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-003-0712-8