Virgibacillus koreensis sp. nov., a novel bacterium from a salt field, and transfer of Virgibacillus picturae to the genus Oceanobacillus as Oceanobacillus picturae comb. nov. with emended descriptions

• Published in: International journal of systematic and evolutionary microbiology Vol. 56; no. 1; pp. 251 - 257
• Main Authors: Lee, Jung-Sook, Lim, Jee-Min, Lee, Keun Chul, Lee, Jae-Chan, Park, Yong-Ha, Kim, Chang-Jin
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.01.2006; Society for General Microbiology
• Subjects: Bacteriology; Biological and medical sciences; Cyanoacrylates; Fatty Acids; Fundamental and applied biological sciences. Psychology; Gram-Positive Endospore-Forming Bacteria - chemistry; Gram-Positive Endospore-Forming Bacteria - classification; Gram-Positive Endospore-Forming Bacteria - isolation & purification; Gram-Positive Endospore-Forming Bacteria - physiology; Hydrogen-Ion Concentration; Korea; Marine; Microbiology; Molecular Sequence Data; Oceanobacillus picturae; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Sequence Homology, Nucleic Acid; Soil Microbiology; Species Specificity; Systematics; Temperature; Virgibacillus koreensis; Korea; INW, Huanghai Sea; Taxonomic review; Salt; Description; Taxonomy
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.63734-0

Korea Research Institute of Bioscience and Biotechnology, 52 Oeundong, Yusong, Daejeon 305-333, Korea Correspondence Chang-Jin Kim changjin{at}kribb.re.kr A novel Virgibacillus strain, BH30097 T , was isolated from a salt field near Taean-Gun on the Yellow Sea in Korea. Cells were Gram-positive rods and bore ellipsoidal endospores in terminal positions. The optimum pH and temperature for growth of this organism were pH 7 and 25 °C, respectively. The main respiratory quinone was MK-7 and the major cellular fatty acids were anteiso-C 15 : 0 and iso-C 16 : 0 . Analysis based on 16S rRNA gene sequence data revealed that the isolate formed an evolutionary lineage distinct from other Virgibacillus species. Levels of sequence similarity between the isolate and other Virgibacillus species ranged from 93·8 to 96·7 %. DNA–DNA relatedness values between strain BH30097 T and a phylogenetically closely related strain, Virgibacillus halodenitrificans KCTC 3790 T , were less than 24 %. On the basis of morphological, physiological and chemotaxonomic characteristics, 16S rRNA gene sequence comparison and DNA–DNA hybridization, a novel species, Virgibacillus koreensis sp. nov., is proposed, with the type strain BH30097 T (=KCTC 3823 T =JCM 12387 T ). It is also proposed that Virgibacillus picturae be transferred to the genus Oceanobacillus as Oceanobacillus picturae comb. nov. based on its 16S rRNA gene sequences and other taxonomic characteristics. Published online ahead of print on 16 September 2005 as DOI 10.1099/ijs.0.63734-0. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain BH30097 T is AY616012 . A photomicrograph showing sporangia and vegetative cells of the type strain of Virgibacillus koreensis is available as supplementary material in IJSEM Online.