Proteomic map of Trypanosoma cruzi CL Brener: the reference strain of the genome project

• Published in: Archives of microbiology Vol. 191; no. 2; pp. 177 - 184
• Main Authors: Sodré, Cátia Lacerda, Chapeaurouge, Alexander D, Kalume, Dário Eluan, de Mendonça Lima, Leila, Perales, Jonas, Fernandes, Octavio
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.02.2009; Springer-Verlag; Springer; Springer Nature B.V
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Cell Biology; Chagas disease; Ecology; Electrophoresis; Electrophoresis, Gel, Two-Dimensional; Fundamental and applied biological sciences. Psychology; Genome, Protozoan; Genomes; Genotypes; Infections; Kinases; Life cycle. Host-agent relationship. Pathogenesis; Life Sciences; Mass spectrometry; Microbial Ecology; Microbiology; Miscellaneous; Molecular Sequence Data; Parasites; Proteins; Proteomics; Protozoa; Protozoan Proteins - chemistry; Protozoan Proteins - genetics; Protozoan Proteins - metabolism; Scientific imaging; Short Communication; Trypanosoma cruzi - chemistry; Trypanosoma cruzi - genetics; Trypanosoma cruzi - metabolism; Brazil; United States > US; Rio de Janeiro Brazil; CL Brener strain; Epimastigote; Proteome; Kinetoplastida; Protozoa; Proteomics; Genome; Strain; Trypanosoma cruzi
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-008-0439-6

In this work two-dimensional gel electrophoresis combined with mass spectrometry was carried out in order to start the construction of a map of soluble proteins from epimastigote form of Trypanosoma cruzi CL Brener. This strain is a hybrid organism derived from two genotypes, T. cruzi I and T. cruzi II and was chosen for genome sequencing. The two-dimensional gel electrophoresis showed that most of proteins focused at 4-7 pH range. The identification demonstrated that several proteins were in multiple isoforms, such as tubulin and heat shock proteins. Potential targets for development of chemotherapeutic agents like arginine kinase, an enzyme absent from mammalian tissues that is involved in the energy supply of the parasite, were also detected.