Bacillus arenosi sp. nov., Bacillus arvi sp. nov. and Bacillus humi sp. nov., isolated from soil

• Published in: International journal of systematic and evolutionary microbiology Vol. 55; no. 1; pp. 111 - 117
• Main Authors: Heyrman, Jeroen, Rodriguez-Diaz, Marina, Devos, Joke, Felske, Andreas, Logan, Niall A, De Vos, Paul
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.01.2005; Society for General Microbiology
• Subjects: Bacillus - chemistry; Bacillus - classification; Bacillus - genetics; Bacillus - isolation & purification; Bacillus arenosi; Bacillus arvi; Bacillus carboniphilus; Bacillus pycnus; Bacillus sporothermodurans; Bacteriology; Biological and medical sciences; DNA Fingerprinting - methods; DNA, Bacterial - analysis; DNA, Ribosomal - analysis; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Genome, Bacterial; Microbiology; Molecular Sequence Data; Netherlands; Nucleic Acid Hybridization; Phenotype; Phylogeny; Polymerase Chain Reaction; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Systematics; Netherlands; Soils; Bacillales; Taxonomy; Bacillus; Bacteria; New species; Bacillaceae
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.63240-0

1 Vakgroep BFM WE10V, Laboratorium voor Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium 2 School of Biological and Biomedical Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow G4 0BA, UK 3 GBF (German Research Centre of Biotechnology), Division of Microbiology, Mascheroder Weg 1, D-38124 Braunschweig, Germany Correspondence Jeroen Heyrman Jeroen.Heyrman{at}UGent.be A group of nine Gram-positive endospore-forming bacteria was isolated from soil of the Drentse A agricultural research area in the Netherlands. Using (GTG) 5 -PCR genomic fingerprinting and fatty acid analysis, the nine isolates were divided into three consistent groups. On the basis of 16S rRNA gene sequence similarity of representative strains, the nine isolates were shown to belong to the genus Bacillus . The first group of four isolates was most closely related to Bacillus carboniphilus (95·5 %) and Bacillus sporothermodurans (95·5 %). The second and third groups of three and two isolates, respectively, showed highest sequence similarity to Bacillus neidei (97·0 and 97·1 %, respectively) and Bacillus pycnus (both 96·7 %). A DNA–DNA relatedness study confirmed the consistency of the three groups delineated by (GTG) 5 -PCR and fatty acid analysis. A small number of phenotypic characters allowed differentiation of the three groups of isolates. The three groups therefore represent novel species, for which the names Bacillus humi , Bacillus arenosi and Bacillus arvi are proposed, with LMG 22167 T (=DSM 16318 T ), LMG 22166 T (=DSM 16319 T ) and LMG 22165 T (=DSM 16317 T ) as the respective type strains. Published online ahead of print on 20 August 2004 as DOI 10.1099/ijs.0.63240-0. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequences of LMG 22165 T , LMG 22166 T , LMG 22167 T and LMG 22168 are AJ627211 , AJ627212 , AJ627210 and AJ627209 , respectively. The sporangial morphologies of B. arenosi and B. humi are provided as supplementary material in IJSEM Online.