Protective Effect of Probiotics against Pseudomonas aeruginosa Infection of Human Corneal Epithelial Cells
Probiotic therapy needs consideration as an alternative strategy to prevent and possibly treat corneal infection. This study aimed to assess the preventive effect of and subsp. on reducing the infection of human corneal epithelial (HCE) cells caused by . The probiotics' preventive effect agains...
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Published in | International journal of molecular sciences Vol. 25; no. 3; p. 1770 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
MDPI AG
01.02.2024
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Subjects | |
Online Access | Get full text |
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Summary: | Probiotic therapy needs consideration as an alternative strategy to prevent and possibly treat corneal infection. This study aimed to assess the preventive effect of
and
subsp.
on reducing the infection of human corneal epithelial (HCE) cells caused by
. The probiotics' preventive effect against infection was evaluated in cell monolayers pretreated with each probiotic 1 h and 24 h prior to
challenge followed by 1 h and 24 h of growth in combination. Cell adhesion, cytotoxicity, anti-inflammatory, and antinitrosative activities were evaluated.
and
adhered to HCE cells, preserved occludin tight junctions' integrity, and increased mucin production on a SkinEthic
HCE model. Pretreatment with
or
significantly protected HCE cells from infection at 24 h, increasing cell viability at 110% (110.51 ± 5.15;
≤ 0.05) and 137% (137.55 ± 11.97;
≤ 0.05), respectively. Each probiotic showed anti-inflammatory and antinitrosative activities, reducing TNF-α level (
≤ 0.001) and NO
amount (
≤ 0.001) and reestablishing IL-10 level (
≤ 0.001). In conclusion, this study demonstrated that
and
exert protective effects in the context of corneal infection caused by
by restoring cell viability and modulating inflammatory cytokine release. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1422-0067 1661-6596 1422-0067 |
DOI: | 10.3390/ijms25031770 |