Glucose transport in carp erythrocytes: individual variation and effects of osmotic swelling, extracellular pH and catecholamines

The characteristics of the uptake of 3-O-methyl-d-glucose (3-OMG) by carp (Cyprinus carpio) erythrocytes were studied in vitro with tracer methods. There is large individual variation in the permeability of the carp erythrocyte membrane to 3-OMG. Although transport is inhibited by cytochalasin B and...

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Bibliographic Details
Published inJournal of experimental biology Vol. 198; no. Pt 2; pp. 577 - 583
Main Authors Tiihonen, K, Nikinmaa, M, Lappivaara, J
Format Journal Article
LanguageEnglish
Published England The Company of Biologists Ltd 01.02.1995
Subjects
Cellular biology
Cyprinus carpio
Fish
Freshwater
Metabolism
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Summary:The characteristics of the uptake of 3-O-methyl-d-glucose (3-OMG) by carp (Cyprinus carpio) erythrocytes were studied in vitro with tracer methods. There is large individual variation in the permeability of the carp erythrocyte membrane to 3-OMG. Although transport is inhibited by cytochalasin B and phloretin, the lack of saturation kinetics for transport in a physiologically relevant concentration range suggests either that a glucose transporter does not exist or that its affinity for glucose is extremely low. The marked increase in transport after osmotic swelling and the inhibition of swelling-induced glucose transport by cytochalasin B suggest that the glucose transport pathway in carp erythrocytes (both in isotonic and hypotonic conditions) may be similar to the volume-activated channel described for flounder erythrocytes. 3-OMG transport across the carp erythrocyte membrane is increased by catecholamines by a mechanism independent of the catecholamine-induced cell swelling.
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ISSN:0022-0949
1477-9145
DOI:10.1242/jeb.198.2.577