Variations in photoperiods and their impact on yield, photosynthesis and secondary metabolite production in basil microgreens

• Published in: BMC plant biology Vol. 24; no. 1; pp. 712 - 13
• Main Authors: Fayezizadeh, Mohammad Reza, Ansari, Naser Alemzadeh, Sourestani, Mohammad Mahmoodi, Hasanuzzaman, Mirza
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 26.07.2024; BioMed Central; BMC
• Subjects: Analysis; Anthocyanin; Anthocyanins; Antioxidant capacity; Antioxidants; Antioxidants - metabolism; Ascorbic acid; Basil; Carbon dioxide; Chlorophyll; Continuous lighting; Cultivars; Diseases and pests; Environmental factors; Flavonoids; Genetic aspects; Genotype; Genotypes; Growth; Health aspects; Health promotion; Herbal medicine; Humidity; Identification and classification; Leaves; Light; Medicinal plants; Metabolites; Ocimum basilicum - genetics; Ocimum basilicum - growth & development; Ocimum basilicum - metabolism; Ocimum basilicum L; Performance evaluation; Phenolic compounds; Phenols; Photoperiod; Photoperiodicity; Photoperiodism; Photosynthesis; Photosynthetic pigments; Phytochemicals; Plant metabolites; Plants (botany); Proline; Secondary Metabolism; Secondary metabolites; Stress; Vegetables; Vitamin C; South Korea; Iran; Antioxidant capacity; Phenols; Continuous lighting; Stress; Ocimum basilicum L; Vitamin C
• ISSN: 1471-2229; 1471-2229
• DOI: 10.1186/s12870-024-05448-z

The effects of different photoperiods on plant phytochemical synthesis can be improved by adjusting the daily light integral. Photoperiod is one of the most important environmental factors that control growth, plant's internal rhythm and the synthesis of secondary metabolites. Information about the appropriate standard in terms of photoperiod for growing basil microgreens as one of the most important medicinal plants is limited. In this study, the effects of five different photoperiods, 6 (6 h × 3 cycles), 8 (8 h × 2 cycles), 16, 18, and 24 h day on the yield, photosynthesis and synthesis of secondary metabolites of three cultivars and one genotype of basil microgreens in floating system were evaluated. The purpose of this research was to determine the feasibility of using permanent light in growing basil microgreens and to create the best balance between beneficial secondary metabolites and performance. The results showed that the effects of photoperiod and cultivar on all investigated traits and their interaction on photosynthetic pigments, antioxidant capacity, total phenolic compounds, proline content and net photosynthesis rate were significantly different at the 1% level. The highest levels of vitamin C, flavonoids, anthocyanins, yield and antioxidant potential composite index (APCI) were obtained under the 24-h photoperiod. The highest antioxidant capacity was obtained for the Kapoor cultivar, and the highest total phenolic compound and proline contents were measured for the Ablagh genotype under a 24-h photoperiod. The highest yield (4.36 kg m ) and APCI (70.44) were obtained for the Ablagh genotype. The highest nitrate content was obtained with a photoperiod of 18 h for the Kapoor cultivar. The highest net photosynthesis rate was related to the Violeto cultivar under a 24-hour photoperiod (7.89 μmol CO m s ). Antioxidant capacity and flavonoids had a positive correlation with phenolic compounds and vitamin C. Yield had a positive correlation with antioxidant capacity, flavonoids, vitamin C, APCI, and proline. Under continuous light conditions, basil microgreens resistance to light stress by increasing the synthesis of secondary metabolites and the increase of these biochemical compounds made basil microgreens increase their performance along with the increase of these health-promoting compounds. The best balance between antioxidant compounds and performance was achieved in continuous red + blue light. Based on these results, the use of continuous artificial LED lighting, due to the increase in plant biochemical with antioxidant properties and yield, can be a suitable strategy for growing basil microgreens in floating systems.