Autophagy-regulated AMPAR subunit upregulation in in vitro oxygen glucose deprivation/reoxygenation-induced hippocampal injury

• Published in: Brain research Vol. 1668; pp. 65 - 71
• Main Authors: Bao, Li, Li, Rong-Hu, Li, Mei, Jin, Mei-Fang, Li, Gang, Han, Xing, Yang, Yuan-Yuan, Sun, Bin, Xu, Li-Xiao, Feng, Xing
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2017
• Subjects: Adenine - analogs & derivatives; Adenine - pharmacology; AMPAR; Animals; Autophagy; Autophagy - drug effects; Brain Ischemia - metabolism; Cell Hypoxia - drug effects; Cell Survival - drug effects; Cells, Cultured; Glucose - metabolism; Hippocampal neuron; Hippocampus - drug effects; Hippocampus - injuries; Hippocampus - metabolism; Neurology; Neurons - metabolism; Oxygen - metabolism; Oxygen glucose deprivation; Rats, Sprague-Dawley; Receptors, AMPA - metabolism; Reoxygenation; Reperfusion Injury - metabolism; Transcriptional Activation - drug effects; Oxygen glucose deprivation; Hippocampal neuron; Reoxygenation; Autophagy; AMPAR
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2017.05.019

Highlights • OGD/R-induced hippocampal neuron injury. • OGD/R-induced neuron injury was accompanied by accumulation of autophagosomes and autolysosomes in cytoplasm alongside a dramatic increase in expression of autophagy-related genes, LC3 and Beclin 1 and increased intracellular Ca2+ levels. • Pre-treatment with autophagy inhibitor 3-MA significantly reduced autophagy. • OGD/R-induced upregulation of AMPAR subunits (GluR1, GluR1, and GluR3) was effectively reversed in cells pretreated with 3-MA.